Analiza eteričnih olj izbranih vrst rutičevk (Rutaceae), rožnic (Rosaceae) in lanikovk (Santalaceae)

Krajnc, Ema (avtor), Kočevar Glavač, Nina (mentor)

Eterična olja so kompleksne zmesi hlapnih spojin, ki jih pridobivamo iz aromatičnih rastlin oz. njihovih delov. So močnega vonja, zelo hlapna in večinoma brezbarvna. Njihova uporaba pri zdravljenju bolezni sega daleč v preteklost in se je prenašala iz roda v rod, danes pa je poznana kot aromaterapija. Čeprav so narejene številne raziskave o delovanju eteričnih olj ali njihovih sestavin, točnih mehanizmov večinoma ne poznamo. V magistrski nalogi smo proučevali izbrana eterična olja iz družin rutičevk (Rutaceae), rožnic (Rosaceae) in lanikovk (Santalaceae) z metodo plinske kromatografije, sklopljene z masno spektrometrijo (GC-MS). Identificirali in kvantificirali smo spojine v posameznih eteričnih oljih. Ugotavljali smo razlike v sestavi eteričnih olj glede na geografsko lego rastlin in vrsto rastline. V drugem delu smo raziskali literaturne podatke o delovanju eteričnih olj iz in vitro, in vivo in kliničnih raziskav ter podatke o delovanju posameznih spojin, da bi ugotovili, katere spojine so odgovorne za njihovo delovanje. Z analizo GC-MS smo ugotovili, da večina eteričnih olj vsebuje dve do tri glavne komponente v vsebnosti več kot 10 %, ostale spojine, ki so prisotne v manjši koncentraciji, pa so zelo raznolike. Vsebnost prisotnih spojin se lahko glede na izvor in vrsto rastline spreminja. Večina proučevanih eteričnih olj deluje protimikrobno, protiglivno, antioksidativno in protivnetno. Največ različnih delovanj smo ugotovili pri eteričnih oljih damaščanske vrtnice in belega santalovca. Glede na vsebnost določenih spojin v eteričnem olju in podatke o delovanju spojin lahko sklepamo, da so delovanja eteričnih olj posledica sinergističnih oz. antagonističnih učinkov posameznih spojin. V večini primerov delovanje pripišemo več spojinam v eteričnem olju ali pa povezav nismo našli. Na delovanje eteričnega olja zato le na podlagi vsebnosti določenih spojin ne moremo sklepati. V prihodnosti bi bilo potrebno opraviti še dodatne raziskave, predvsem klinične, da bi omogočili varno terapevtsko uporabo.

• Jezik: Slovenski jezik
• Ključne besede: eterična olja, analiza GC-MS, sestava, delovanje eteričnih olj, klinična raziskava
• Vrsta gradiva: Magistrsko delo/naloga
• Organizacija: FFA - Fakulteta za farmacijo
• Leto izida: 2019
• PID: 20.500.12556/RUL-107721
• Datum objave v RUL: 17.05.2019

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Analysis of essential oils from selected rue (Rutaceae), rose (Rosaceae) and sandalwood (Santalaceae) species

Izvleček

Essential oils are complex mixtures of compounds produced in aromatic plants. They are volatile, mostly colourless liquids with strong odour. The use of essential oils in the treatment of diseases dates back to the past and has been passed from generation to generation. Today it is known as aromatherapy. Although numerous studies have been done, the exact mechanisms of action of essential oils or their constituents are largely unknown. In this work, we studied the selected essential oils of the rue family (Rutaceae), rose family (Rosaceae) and sandalwood family (Santalaceae) by the gas chromatography-mass spectrometry (GC-MS) method. We identified and quantified the compounds in essential oils. We focused on differences in the composition of essential oils, depending on the geographical location and the species of the plant. In the second part of the master's thesis, we studied data on the activity of essential oils based on in vitro, in vivo and clinical studies, and data on the activity of individual compound in order to determine the compounds which may be responsible for the activity of essential oils. Using the GC-MS method, it has been found that the majority of essential oils contain two to three main components in concentrations higher than 10 %. The other compounds are present in lower concentration and are very diverse. The content of the compounds in the plant may vary according to the plant origin and the species of the plant. Most of the studied essential oils have antimicrobial, antifungal, antioxidant and anti-inflammatory activity. In the damask rose essential oils and sandalwood essential oils we found the most various effects. Depending on the concentrations of certain compounds in essential oils and on the data about the activity of the compounds, it can be concluded that the effects of essential oils are probably a consequence of the synergistic or antagonistic effects of individual compounds. In most cases, the activity is associated to several compounds in an essential oil or no connections were found between the effects of an essential oil and compounds. Therefore, we can not deduce the effect of an essential oil based on the content of certain compounds. In the future, additional research, especially clinical, should be made to enable safe therapeutic use.

• Ključne besede: essential oils, GC-MS analysis, composition, activity of essential oils, clinical research