Spin labeling is a method of attaching stable paramagnetic molecules to the material of investigation in order to obtain selective structural and dynamic information with electron paramagnetic resonance, a method for the detection of unpaired electrons. By incorporating spin probes into the selected system and studying radicals with electron paramagnetic resonance, we can obtain information about membrane modeling systems and biological membranes.
We have synthesized seven spin probes, which contain the cyclic nitroxide radical and planar aromatic structure in their structure. After synthesis, the reaction products were purified and separated by column flash chromatography. New compounds were characterized by mass spectrometry, infrared spectroscopy and electron paramagnetic resonance.
We have shown that the synthesis of nitroxide spin probes is not difficult and gives good yields and few by-products.
Results are important starting points for further optimization of the synthesis of spin probes. These can be used for future studies of carbon nanotubes attached to spin probes in biological systems, using electron paramagnetic resonance, monitoring their dynamics and checking possible cytotoxic and cytoprotective effects.
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