The catalytic domain of cathepsin C (dipeptidyl-peptidase I) alone is a fully functional endoprotease

Rebernik, Mateja (author), Lenarčič, Brigita (author), Novinec, Marko (author)

Cathepsin C is a tetrameric lysosomal protease that acts as a dipeptidyl-peptidase due to the presence of the exclusion domain that is unique among papain-like cysteine proteases. Here we describe a recombinant form of cathepsin C lacking its exclusion domain (CatCΔEx) produced in a bacterial expression system (E. coli). CatCΔEx is a monomer with endoprotease activity and affinity for hydrophobic residues such as Phe, Leu or Pro, but not Val, in the P2 position. As opposed to cathepsin C, it does not require chloride ions for its activity. Despite lower turnover rates of hydrolysis of synthetic substrates, CatCΔEx has elastolytic and gelatinolytic activity comparable to other cysteine cathepsins.

• Language: English
• Keywords: proteolysis, oligomeric proteins, exclusion domain, elastolysis, gelatinolysis
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Author Accepted Manuscript
• Year: 2019
• Number of pages: Str. 21-27
• Numbering: Vol. 157
• PID: 20.500.12556/RUL-106687
• UDC: 577.15
• ISSN on article: 1046-5928
• DOI: 10.1016/j.pep.2019.01.009
• COBISS.SI-ID: 1538119107
• Publication date in RUL: 12.03.2019

Record is a part of a journal

• Title: Protein expression and purification
• Shortened title: Protein expr. purif.
• Publisher: Elsevier, Academic Press
• ISSN: 1046-5928
• COBISS.SI-ID: 2627623

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
• Licensing start date: 12.03.2019

Secondary language

• Language: Slovenian
• Keywords: proteoliza, oligomerni proteini, izključitvena domena, elastoliza, želatinoliza

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0140
• Name: Proteoliza in njena regulacija