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Probiotična prehranska dopolnila v luči spremenjene zakonodaje o zdravstvenih trditvah na živilih
ID Sikošek, Miha (Author), ID Bogovič Matijašić, Bojana (Mentor) More about this mentor... This link opens in a new window, ID Mohar Lorbeg, Petra (Comentor)

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Abstract
Število probiotičnih izdelkov tako na slovenskem kot tudi svetovnem trgu vsako leto strmo narašča. Raziskave kakovosti in ustreznosti probiotičnih prehranskih dopolnil pogosto pokažejo neskladnost med deklariranimi in ugotovljenimi podatki o številu in vrsti probiotičnih bakterij v izdelkih. Razen tega neredko poročajo o zdravstvenih trditvah, ki jih na probiotičnih prehranskih dopolnilih ne bi smelo biti. Namen magistrskega dela je bil analizirati nekatere probiotične izdelke, ki so naprodaj v lekarnah in specializiranih trgovinah na slovenskem trgu. Pod drobnogled smo vzeli 19 izdelkov ter s pomočjo konvencionalne metode štetja na selektivnih gojiščih in molekularno-bioloških metod preverili skladnost glede števila in prisotnosti deklariranih bakterij. Prav tako smo preverili, ali imajo izdelki ustrezne deklaracije. Na vseh izdelkih so bile navedene bakterijske vrste, ne pa tudi posamezni sevi. Na večini deklaracij so bili le podatki za skupno število probiotičnih bakterij, ne pa za posamezno bakterijsko vrsto. Le devet od devetnajstih izdelkov je vsebovalo zadostno število bakterij in le osem izdelkov je vsebovalo vse deklarirane bakterijske vrste. Prisotnost deklariranih bakterijskih vrst smo ugotavljali z metodo PCR. Standardne gojitvene metode zaradi omejenega izbora selektivnih gojišč večinoma ne omogočajo razlikovanja med posameznimi bakterijskimi vrstami, so pa uporabne za ugotavljanje skupnega števila probiotičnih bakterij. Domneva, da so na slovenskem trgu probiotični izdelki, ki ne ustrezajo kriterijem kakovosti in ustreznosti, se je izkazala za upravičeno.

Language:Slovenian
Keywords:probiotiki, prehranska dopolnila, označevanje izdelkov, deklaracije izdelkov, zakonodaja, probiotične bakterije, mikrobiološke metode, varnost potrošnika
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Publisher:[M. Sikošek]
Year:2018
PID:20.500.12556/RUL-105887 This link opens in a new window
UDC:579.67:615.372:577.2.083
COBISS.SI-ID:5011576 This link opens in a new window
Publication date in RUL:22.12.2018
Views:3056
Downloads:706
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Secondary language

Language:English
Title:Probiotic dietary supplements in the light of the changed legislation on health claims on foods
Abstract:
The number of probiotic products on Slovenian and global market is increasing every year. Research on the quality and adequacy of probiotic food supplements often shows many discrepancies between the number and identity of probiotic bacteria present in the product and those claimed on the label. Often, there have also been health claims observed, which are not allowed on probiotic food supplements. The purpose of this study was to analyze some of the probiotic products avaliable in pharmacies and specialised stores on the Slovenian market. We examined 19 products and used conventional plate counting and molecular methods in order to verify if the number of bacteria corresponds to the number on the label and if declared species are present in the product. We have also checked whether the declarations on the products were appropriate. Names of bacterial species were labeled on all products, however many of them lacked the designation of the bacterial strain. Most of the products had only the total number of bacteria listed but not also the number of each species. Only nine out of nineteen products contained sufficient total number of probiotic bacteria and only eight products contained all of the claimed bacterial species. We used PCR method to detect individual bacterial species. Conventional methods due to the lack of selective growth media often do not allow differentiation between related bacterial speices but are more usefull for the enumeration of total probiotic bacteria. Our hypothesis that there are probiotic products on Slovenian market which doesn´t meet criteria of quality and adequacy, was confirmed.

Keywords:probiotics, food supplements, product labeling, product declarations, legislation, probiotic bacteria, microbiological methods, consumer safety

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