V okviru magistrske naloge smo preučevali vpliv načina osvetljevanja na antioksidativni potencial (AOP) kalčka in preostalega dela kaljenega pšeničnega zrna. Kaljenje je potekalo 4 dni pri 20 °C pod LED lučmi (470 in 520 nm) oz. v temi (kontrola). Po 48 in 96 urah smo kalček ločili od preostalega zrna in pripravili metanolne ekstrakte, preostanek po ekstrakciji pa hidrolizirali z NaOH. S Folin Ciocalteu metodo smo kalčku in preostalemu delu zrna določili vsebnost ekstraktibilnih in vezanih fenolnih spojin, z metodama DPPH in ABTS pa njihov AOP. Intenzivnejše spremembe so potekale v kalčku kot v preostalem zrnu. Uporaba modrih in zelenih LED je vodila k manjši vsebnosti ekstraktibilnih fenolnih spojin in nižjemu AOP ekstraktibilnih antioksidantov tako v zrnih kot kalčkih v primerjavi s temo, medtem ko način osvetljevanja na vsebnost vezanih fenolnih spojin ni imel vpliva. AOP vezanih antioksidantov v zrnih, določen z ABTS metodo, je bil le po 48 urah kaljenja pod LED nižji kot v temi, sicer pa način osvetljevanja zrn in kalčkov ni vplival na AOP ne glede na metodo določanja. Čas kaljenja je statistično značilno vplival na vsebnost ekstraktibilnih fenolnih spojin v zrnih in kalčkih ter na njihov AOP, na vsebnost vezanih fenolnih spojin pa samo v kalčkih, ki so rastli v temi. Z metodo DPPH smo vsem vzorcem določili nižji AOP kot z metodo ABTS.
| Language: | English |
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| Title: | The effect of light of different wavelenght on antioxidant potencial of germinated wheat |
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| Abstract: |
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We studied the effect of type of light on antioxidant potential (AOP) of sprouts and the rest of the germinated wheat grains. Wheat grains were germinated for 4 days at 20 °C where LED lights (470 in 520 nm) presented source of light. Germination in darkness presented control. We took samples after 48 and 96 hours of germination and separated sprouts from the grains and than prepared methanolic extracts. The bound phenolic compounds were released from insoluble residues after hydrolysis with NaOH. Content of extractable and bound phenolic compounds was determined with Folin Ciocalteau method, AOP with DPPH and ABTS methods. More intensive changes were observed in sprouts than in the rest of the grains. Use of blue and green LED contributed to the lower extractable phenolic content and their AOP in grains and sprouts compared to control. Type of LED light did not influence the bound phenolic content. AOP of bound phenolics (ABTS method) was lower after 48 hours of germination under LED light than in control. Otherwise type of light did not effect on AOP of grains and sprouts regardless of type of method. Time of germination significantly effected the content of extractable phenolic compounds in grains and sprouts and their AOP regardless of type of light. Furthermore, time of germination effected on bound phenolic compounds only in sprouts which were grown in dark. For all samples higher AOP was determined with ABTS in comparison to DPPH method.
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| Keywords: | wheat, Triticum aestivum, germination, germinated seeds, sprouts, light, lighting, LED, darkness, phenolic compounds, antioxidant potencial, Folin Ciocalteu method, DPPH method, ABTS method |
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