Učinkovitost konvencionalnih in naravnih čistilnih sredstev na osnovi rastlinskih izvlečkov pri nadzoru bakterijske adhezije : magistrsko delo

Golob, Jernej (author), Fink, Rok (mentor), Oder, Martina (co-mentor)

Uvod: Mikroorganizmi adherirani na površinah lahko predstavljajo vir kontaminacije, ter s tem predstavljajo tveganje za zdravje ljudi. V živilski industriji so pogosto vzrok za kvarjenje živil. Namen: Namen naše raziskave je primerjati učinkovitost konvencionalnih in rastlinskih čistilnih sredstev za odstranjevanja bakterijskih celic E. coli s površine stekla. Rezultate učinkovitosti odstranjevanja mikroorganizmov smo primerjali s podatki o toksičnosti za posamezno čistilno sredstvo. Metode dela: S pomočjo svetlobnega mikroskopa in kamere smo analizirali število bakterijskih celic na površini stekla pred izpostavljenostjo čistilnemu sredstvu, ter po delovanju čistilnega sredstva, brez uporabe fizične sile. Rezultati: Konvencionalna čistilna sredstva so bila glede odstranjevanja bakterijskih celic s površine učinkovitejša, saj smo z njihovo uporabo odstranili od 20 do 45 % celic. Rastlinska čistilna sredstva so odstranila le 4 do 18 % bakterijskih celic E. coli s površine. Učinkovitost čistilnih sredstev je bila odvisna tudi od koncentracije čistilnega sredstva. Razprava in sklep: Konvencionalna čistilna sredstva so bila pri odstranjevanju bakterijskih celic učinkovitejša. Vsa čistilna sredstva so bila najučinkovitejša pri najvišji, 20 % koncentraciji. Najučinkovitejše čistilno sredstvo, ni bilo hkrati akutno najbolj toksično. Ugotovili smo tudi, da akutno najmanj toksično čistilno sredstvo ni bilo zmeraj najmanj učinkovito.

• Language: Slovenian
• Keywords: adhezija, E. coli, konvencionalna čistilna sredstva, naravna čistilna sredstva
• Work type: Master's thesis/paper
• Organization: ZF - Faculty of Health Sciences
• Year: 2018
• PID: 20.500.12556/RUL-105481
• COBISS.SI-ID: 5541995
• Publication date in RUL: 01.12.2018

Secondary language

• Language: English
• Title: Efficiency of conventional and natural cleaning agents based on plant extracts for controlling bacterial adhesion : master thesis

Abstract

Introduction: Microorganisms adhered on surfaces can represent a source of contamination and consequently pose a health risk for humans. In the food industry they are a common cause of food spoilage. Purpose: The purpose of our research was to compare the efficacy of conventional and plant-based cleaning agents for removing E. coli bacterial cells from a glass surface. We compared the efficacy of removing microorganisms with the toxicity index of each individual cleaning agent used. Methods: Using a microscope we count the number of bacterial cells on the surface of the glass before the glass was exposed to the cleaning agent and then after the exposure to the cleaning agent. No physical force was used. Results: Conventional cleaning agents were more effective in removing bacterial cells, removing between 20 % and 45 % of bacterial cells. Plant-based cleaning agents removed between 4 % and 18 % of bacterial cells. The effectiveness of the cleaning agent also depends on the used concentration. Discussion and conclusion: Conventional cleaning agents are more effective in removing bacterial cells. All cleaning agents were most effective at the highest tested concentration of 20 %. The most effective cleaning agent was not the most acute toxic. And the least acute toxic cleaning agent was not the least effective.

• Keywords: adhesion, E. coli, conventional cleaning products, natural based cleaning products