Use of the black soldier fly (Hermetia illucens) for protein production from organic wastes and its microbiome

Bogataj, Tajda (avtor), Stres, Blaž (mentor), Insam, Heribert (komentor)

Larvae of black soldier fly are known biowaste reduction organisms. In this study, larvae were fed with three different substrates (chicken feed-control substrate, food waste and oil waste). Their growth, organic matter, development to prepupae stage, waste reduction and gut microbial community was observed. Oil waste was not suitable substrate for the larvae. On the other hand, food waste and chicken feed were appropriate substrates, which gave heavy and organic rich larvae/prepupae, stimulated larvae development to prepupae stage and reduced wastes. Gut microbiota of the larvae was not affected by substrate microbial community and by the time. Dominant phyla presented were Proteobacteria, Firmicutes and Bacteroidetes.

• Jezik: Angleški jezik
• Ključne besede: wastes, biodegradable wastes, food waste, oil waste, black soldier fly, Hermetia illucens, larvae, prepupae, waste reduction, gut microbiota, sequencing
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Založnik: [T. Bogataj]
• Leto izida: 2018
• PID: 20.500.12556/RUL-104950
• UDK: 579.26:628.4.042
• COBISS.SI-ID: 4972152
• Datum objave v RUL: 18.10.2018

Sekundarni jezik

• Jezik: Slovenski jezik
• Naslov: Uporaba črne vojaške muhe (Hermetia illucens) za pridobivanje proteinov iz organskih odpadkov in analiza njenega mikrobioma

Izvleček

Ličinke črne vojaške muhe (Hermetia illucens; BSF-black soldier fly) so znane razgrajevalke bioloških odpadkov. V tej študiji smo ličinke hranili s tremi različnimi substrati (kokošja krma-kontrolni substrat, ostanki hrane in ostanki olja). Opazovali smo njihovo rast, delež organskih snovi, njihov razvoj v stadij bube, sposobnost razgradnje odpadkov in njihovo mikrobno združbo prebavil. Rezultati so pokazali, da odpadki olja niso primeren substrat za gojenje ličink BSF. Ostala dva substrata (ostanki hrane in kokošja krma) sta bila primerna za gojenje Hermetia illucens. Ličinke so se razvile v težke in organsko bogate zrele ličinke sposobne razgradnje odpadkov. Ostanki hrane in kokošja krma sta spodbudili prehod ličink v naslednji stadij bube. Na mikrobioto prebavil ličinke mikrobiota substratov ni vplivala. Prav tako čas inkubacije ni vplival na mikrobno združbo v prebavilih. Prevladujoča debla so bila Proteobacteria, Firmicutes in Bacteroidetes.

• Ključne besede: odpadki, biološko razgradljivi odpadki, ostanki hrane, odpadno olje, črna vojaška muha, Hermetia illucens, ličinke, bube, razgradnja odpadkov, mikrobiota prebavil, sekvenciranje