Merjenje lomnega količnika bioloških tkiv z Abbejevim refraktometrom

Kočevar, Tilen (author), Milanič, Matija (mentor)

Cilj zaključne naloge je bil izdelati refraktometer, ki bi lahko spektralno izmeril lomne količnike tkiv. Na začetku razložim osnovne metode, na katerih temelji moj sistem. Obravnavam dosedanje metode za merjenje lomnega količnika, ter izdelam svoj sistem za spektralno merjenje. V zaključnem delu naloge izvedem meritve ter podam dobljene rezultate, hkrati pa tudi podam možnosti izboljšav.

• Language: Slovenian
• Keywords: Abbejev refraktometer, spektrometer, tkiva, popolni odboj, lomni količnik
• Work type: Final paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FMF - Faculty of Mathematics and Physics
• Year: 2018
• PID: 20.500.12556/RUL-103707
• COBISS.SI-ID: 3256932
• Publication date in RUL: 23.09.2018

Secondary language

• Language: English
• Title: Measurement of tissue refractive index by Abbe refractometer

Abstract

The goal of the thesis was to build a refractometer that measure spectrally resolved refractive indices of biological tissues. In the first part of the thesis the background of the refractometry and tissue properties are presented. Next, design, specific parts and construction of the refractometer, based on the reflectance Abbe refractometer, are presented. The constructed system was characterized using air and water as samples. Finally, possible improvements of the system are presented. It was demonstrated, that the reflectance Abbe refractometer can be fully automatized and be used to perform spectrally resolved refractometry.

• Keywords: Abbe refractometer, spectrometer, tissue, total reflection, refractive index