Seeds have a very important role in human nutrition and recent studies have shown that germination of seeds improved their nutritional value. In graduate thesis we investigated antioxidant efficacy of industrial hemp (Cannabis sativa L.). The extracts of defatted non-germinated and germinated seeds for 24, 48, 72, 96 h were used. Antioxidant efficacy of extracts was measured with DPPH• radical scavenging method and β-carotene bleaching assay. The results were expressed as percentage of remaining DPPH• radical after 30 min and as extract efficiency against β-carotene bleaching after 120 min. From the DPPH• assay we can conclude that antioxidant efficacy is changing during germination. After 48 h lower extract efficacy is determined when compared to non-germinated seeds (27 %). The highest DPPH• scavenging activity express 96 h germinated seeds (36 %). These results don’t coincide with β-carotene bleaching test; a decrease of antioxidant efficacy for extracts of seeds germinated for 24 h is noticed, meanwhile the highest extract efficacy (61 %) after 72 h of germination is determined. The weak correlation of both methods confirms differences in the antioxidative efficacy of extracts in a homogeneous and heterogeneous environment. It has been shown that the reaction rate decreases with time of incubation in both cases, while there is no differences in kinetics between individual samples.
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