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Primerjava antioksidativne učinkovitosti in skupnih fenolih spojin v zelenem in matcha čaju
ID Štravs, Ana (Author), ID Pogačnik, Lea (Mentor) More about this mentor... This link opens in a new window

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Abstract
V okviru diplomskega dela smo določali antioksidativno učinkovitost (AOU) in vsebnost skupnih fenolnih spojin (SF) v zelenem in matcha čaju. Preverili smo, kako različni načini priprave čajev vplivajo na AOU in vsebnost skupnih fenolnih spojin. Osnovno čajno infuzijo smo pripravili tako, da smo 1,6 g posameznega vzorca čaja prelili z 1 dL vodovodne vode s 70 °C ter vzorce ekstrahirali 3 minute. Nato smo spreminjali različne parametre pri pripravi čaja ter AOU in vsebnost SF tako pripravljenih vzorcev primerjali z osnovnimi vzorci čajev. AOU vzorcev smo določili z metodo lovljenja radikala DPPH•, SF pa smo določili s Folin-Ciocalteujevo metodo. Ugotovili smo, da imajo nekateri načini priprave čajne infuzije velik vpliv na AOU in vsebnost SF v čaju. Določili smo, da daljši čas ekstrakcije pozitivno vpliva tako na vsebnost SF kot na AOU infuzij. Višja temperatura in manjša prostornina vode pozitivno vplivata na vsebnost SF in AOU v infuzijah zelenih čajev ter na AOU infuzije matcha čaja, vendar negativno vplivata na vsebnost SF v infuziji matcha čaja. Podaljšan čas po pripravi nima značilnega vpliva na vsebnost SF in AOU infuzij, uporaba destilirane vode pa ima na večino vzorcev majhen, vendar pozitiven vpliv. Glede na skupne rezultate vseh analiz pri pripravi čajnih infuzij lahko zaključimo, da imajo najvišjo AOU in največjo vsebnost SF infuzije matcha čaja, vrednost AOU ter vsebnost SF pa najlažje povečamo tako, da podaljšamo ekstrakcijski čas ali pa povišamo temperaturo vode za pripravo infuzije zelenega čaja.

Language:Slovenian
Keywords:fenolne spojine, antioksidanti, antioksidativna učinkovitost, zeleni čaj, matcha čaj, Folin-Ciocalteu, DPPH•
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Publisher:[A. Štravs]
Year:2018
PID:20.500.12556/RUL-101848 This link opens in a new window
UDC:663.95:547.56:66.094.3.097.8
COBISS.SI-ID:4928888 This link opens in a new window
Publication date in RUL:08.07.2018
Views:10505
Downloads:554
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Secondary language

Language:English
Title:Comparison of antioxidant activity and total phenols in green tea and matcha tea
Abstract:
In the course of this graduation thesis we compared antioxidant capacity (AOC) and total phenolic compounds (TP) in green tea and matcha tea. We have examined how different methods of preparing tea infusions affect AOC and TP. The basic tea infusion was prepared with 1.6 g tea sample and 1 dL of tap water at 70 °C and we extracted samples for 3 minutes. Then we changed the parameters of tea preparation and compared AOC and TP of such samples with basic tea infusions. AOC of the samples was determined by DPPH• assay and TP were determined by Folin-Ciocalteu method. We have found that some methods of preparing a tea infusion have a significant effect on the AOC and TP in the infusions. We have determined that longer extraction time has a positive effect on both the TP content and the AOC of infusions. A higher temperature and a lower volume of water both have a positive effect on the content of TP and AOC in green tea infusions and on the AOC of matcha tea infusion, but have a negative effect on the content of TP in matcha tea infusion. The prolonged time after preparation of the infusions has no specific effect on the content of TP and AOC of infusions, and the use of distilled water has a small, but positive effect on most samples. Based on the overall results of all the analyzes in the preparation of tea infusions, it can be concluded that matcha tea infusions have the highest AOC and the highest content of TP, and that the AOU value and the content of SF are easily increased by extending the extraction time or increasing the water temperature in preparing green tea infusions.

Keywords:phenolic compounds, antioxidants, antioxidant activity, green tea, matcha tea, Folin-Ciocalteu, DPPH•

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