Odziv fenolnih spojin v različnih delih lista žlahtne vinske trte (Vitis vinifera L.) sorte 'Merlot' na virusno bolezen zvijanja listov

Hajdinjak, Tina (avtor), Mikulič Petkovšek, Maja (mentor)

V vinogradu v Goriških Brdih smo na listih trt sorte 'Merlot' opazili atipična bolezenska znamenja virusne bolezni zvijanja listov, ki jo povzročajo virusi GLRaV. Na sedmih trtah smo 28.8.2017 povzorčili liste, od katerih jih je pet trt kazalo atipična znamenja okužbe z GLRaV, ena trta tipična znamenja okužbe z GLRaV-3 in ena trta je bila brez vidnih bolezenskih znamenj, zato smo jo označili kot kontrolo (K). Na desetih listih znotraj vsakega obravnavanja smo s škarjami izrezali žile (Ž) in listno ploskev (L), zato smo jih označili kot GLRaV-2_Ž, GLRaV-2_L, GLRaV-3_Ž, GLRaV-3_L, K_Ž in K_L. Na trtah z atipičnimi bolezenskimi znamenji, to je rdeče obarvanje žil ter packasto rdečenje medžilnega dela listov, smo identificirali GLRaV-2. Iz rezultatov lahko sklepamo, da prisotnost virusne okužbe vpliva na vsebnost fenolnih spojin v Ž in L listov vinske trte, saj smo značilno največ skupnih fenolov izmerili pri GLRaV-2, in sicer 3832 mg/100 g v L in 2534 mg/100 g v Ž. Značilne razlike v vsebnosti skupnih fenolov so bile tudi med GLRaV-2 in GLRaV-3, saj smo v Ž pri GLRaV-2 izmerili 2534 mg/100 g skupnih fenolov in pri GLRaV-3 1374 mg/100 g, kar nakazuje na to, da se trta različno odziva na okužbo z GLRaV-2 ali GLRaV-3. V L GLRaV-2 (3832 mg/100 g) smo izmerili značilno več skupnih in posameznih fenolnih spojin kot v Ž (2534 mg/100 g). Med posameznimi fenolnimi spojinami smo v Ž in L izmerili največjo vsebnost derivatov kvercetina, in sicer pri okužbi z GLRaV-2 (v Ž 1040 mg/100 g in v L 1590 mg/100 g). Značilno največjo vsebnost antocianinov smo izmerili v Ž pri GLRaV-2 (22,9 mg/100 g), medtem ko pri GLRaV-3 (110 mg/100 g) v L. Rezultati potrjujejo, da se fenolne spojine v različnih delih lista različno odzivajo na okužbe virusov, ki povzročajo bolezen zvijanja listov.

• Jezik: Slovenski jezik
• Ključne besede: virus, GLRaV, fenolne spojine, listi, vinska trta, Vitis vinifera
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Založnik: [T. Hajdinjak]
• Leto izida: 2018
• PID: 20.500.12556/RUL-101628
• UDK: 634.8:632.38:547.56(043.2)
• COBISS.SI-ID: 8991609
• Datum objave v RUL: 22.06.2018

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: The response of phenolic compounds in different parts of the grapevine (Vitis vinifera L.) leaf of 'Merlot' variety to the infection with grapevine leafroll disease

Izvleček

Atypical symptoms of grapevine leafroll disease, caused by grapevine leafroll-associated viruses (GLRaVs), were observed on leaves of grapevine cultivar ‘Merlot’, grown in a vineyard in Goriška Brda. On the 28.8.2017, the leaves from seven vines were sampled, five of which exhibited atypical symptoms of GLRaV, one with typical symptoms of GLRaV-3 and one had no visual signs of disease (control or K). Ten leaves per treatment were cut off from leaf veins (Ž) and leaf surface (L) and labelled as: GLRaV-2_Ž, GLRaV-2_L, GLRaV-3_Ž, GLRaV-3_L, K_Ž and K_L. In vines with atypical symptoms (red discoloration of veins and red blotches on leaf surface) GLRaV-2 was identified. Obtained results lead to conclusion that the virus has an impact on the content of phenolic compounds in leaf veins and surface, since the highest content of total analysed phenolics at GLRaV-2 was 3832 mg/100 g in L and 2534 mg/100 g in Ž. Significant differences in the content of total analysed phenolics were between GLRaV-2 and GLRaV-3 in Ž (2534 mg/100 g and 1374 mg/100 g, respectively). In L of GLRaV-2 (3832 mg/100 g) significantly higher content of total and individual phenolic compounds were determined than in Ž (2534 mg/100 g). Among individual phenolics, the highest measured content belonged to derivatives of quercetin, and at GLRaV-2, their contents were 1040 mg/100 g in Ž and 1590 mg/100 g in L. The highest content of anthocyanins was measured in Ž at GLRaV-2 (22.9 mg/100 g), while the highest content at GLRaV-3 belonged to L (110 mg/100 g). The results confirm that phenolic compounds in different parts of the leaves react differently to infection, which causes grapevine leafroll disease.

• Ključne besede: virus, GLRaV, phenolic compounds, leaves, grapevine, Vitis vinifera