Raznolikost antocianov in drugih fenolnih snovi v medvrstnih križancih rodu Sambucus

Todorović, Biljana (author), Štampar, Franc (mentor), Ivančič, Anton (comentor)

Doktorsko delo obravnava doslej malo raziskano variabilnost sekundarnih metabolitov (hidrokscimetnih kislin, flavonolov, flavanonov, flavanolov in antocianov) v ekstraktih socvetij in plodov pri vrstah Sambucus cerulea, S. nigra, S. racemosa in S. javanica ter nekaterih njihovih medvrstnih križancih S. javanica × S. nigra, JA/NI; (S. javanica × S. nigra) × S. nigra, JANI/NI; S. javanica × (S. javanica × S. nigra), JA/JANI; S. javanica × S. cerulea, JA/CER; (S. javanica × S. nigra) × S. cerulea, JANI/CER; S. cerulea × S. nigra, CER/NI; S. javanica × S. racemosa, JA/RAC; (S. javanica × S. nigra) × S. racemosa, JANI/SIB; (S. javanica × S. nigra) × cv. Black Beauty, JANI × cv. Black Beauty; ((S. javanica × S. nigra) × S. nigra) × cv. Black Beauty), JANI/NI × cv. Black Beauty. Analize so pokazale, da so bile razlike med nekaterimi proučevanimi vrstami in medvrstnimi križanci glede vsebnosti in raznolikosti omenjenih sekundarnih metabolitov v ekstraktih socvetij in plodov zelo velike. Za socvetne vodne (čajne) ekstrakte vrste S. nigra je bila značilna velika vsebnost sladkorjev, organskih kislin in fenolov. Največja vsebnost sladkorjev je bila značilna za medvrstni križanec JANI/CER. Največje vsebnosti fenolnih spojin v socvetnih čajnih ekstraktih so bile ugotovljene pri vrsti S. cerulea in medvrsnih križancih JANI × cv. Black Beauty, JANI/CER in JANI/NI. Flavanoli so bili ugotovljeni v metanolnih ekstraktih socvetij pri vrsti S. cerulea in medvrstnem križancu JA/CER. Za ekstrakte plodov vrste S. nigra je bila značilna večja vsebnost skupnih organskih kislin in fenolov. Med medvrstnimi križanci sta imela največjo vsebnost organskih kislin JANI/NI in JA/JANI. Glede skupne vsebnosti vseh antocianov so bili na prvem mestu ekstrakti plodov medvrstnega križanca JA/RAC. Raziskava je pokazala, da so medvrstna križanja zelo popestrila kemično sestavo bezgov, zato bi bilo smiselno tak pristop še naprej uporabljati v njihovem žlahtnjenju.

• Language: Slovenian
• Keywords: Sambucus spp., bezgi, medvrstni križanci, socvetja, bezgove jagode, čajni ekstrakti, antociani, fenolne spojine, sladkorji, organske kisline
• Work type: Doctoral dissertation
• Typology: 2.08 - Doctoral Dissertation
• Organization: BF - Biotechnical Faculty
• Publisher: [B. Todorović]
• Year: 2018
• PID: 20.500.12556/RUL-100100
• UDC: 634.747:631.526.325:547.56:547.973(043.3)
• COBISS.SI-ID: 8950649
• Publication date in RUL: 07.03.2018

Secondary language

• Language: English
• Title: Diversity of anthocyanins and other phenolic compounds among interspecific hybrids of genus Sambucus

Abstract

The presented doctoral thesis deals with so far little studied variability of secondary metabolites (hydroxycinnamic acid, flavonols, flavanones, flavanols and anthocyanins) in aqueous extracts of inflorescences and fruits of some elderberry species (Sambucus cerulea, S. nigra, S. racemosa, S. javanica) and their interspecific hybrids: S. javanica × S. nigra, JA/NI; (S. javanica × S. nigra) × S. nigra, JANI/NI; S. javanica × (S. javanica × S. nigra), JA/JANI; S. javanica × S. cerulea, JA/CER; (S. javanica × S. nigra) × S. cerulea, JANI/CER; S. cerulea × S. nigra, CER/NI; S. javanica × S. racemosa, JA/RAC; (S. javanica × S. nigra) × S. racemosa, JANI/SIB; (S. javanica × S. nigra) × cv. Black Beauty, JANI × cv. Black Beauty; ((S. javanica × S. nigra) × S. nigra) × cv. Black Beauty), JANI/NI × cv. Black Beauty. Analyses have shown big differences between some of the studied species and interspecific hybrids regarding the contents and variability of mentioned secondary metabolites in extracts of inflorescence and fruit. High contents of sugar, organic acids and phenols are found in inflorescence water (tea) extracts of the S. nigra species. The highest contents of sugar are found in interspecific hybrid JANI/CER. The highest contents of phenol compounds in inflorescence water extracts are found in S. cerulea species and in interspecific hybrids JANI × cv. Black Beauty, JANI/CER and JANI/NI. Flavanols were found in methanolic inflorescence extracts of S. cerulea and the interspecific hybrid JA/CER. Fruit extracts of S. nigra were characterised by high contents of total organic acids and phenols. Among the studied interspecific hybrids, the highest contents of organic acids were determined in fruit extracts of JANI/NI and JA/JANI. Considering the total content of all anthocyanins, the first place belonged to JA/RAC. The study showed that interspecific hybridisation had high impact on the diversification of the chemical composition and therefore it would be worthwhile to continue to use it in the future breeding programmes.

• Keywords: Sambucus spp., elder, interspecific hybrids, inflorescences, elderberries, tea extracts, anthocyanins, phenolic compound, sugars, organic acid