Multi-level intersections are considered to be an important factor of road infrastructure. Unfortunately, there has not been enough attention paid to them in the recent years. Considering the fact that on a relatively short distance multi-level intersections include many different elements of road surfaces which can influence road safety, the main aim of the Master’s thesis was set, namely how the choice of traffic-technical characteristics influences road safety in multi-level intersections. By introducing motion of Technical Specifications for Public Roads in February 2002 (TSC 03.343: Multi-level Road Junctions and Interchanges), there were first steps made towards regulating the multi-level intersections. But it went no further than that; therefore the purpose of the Master’s thesis to find sensible suggestions to be included in the final version of the technical specification. Following the introduction of multi-level intersections regarding their use of time, place and purpose, the thesis presents different types of multi-level intersections as well as their main components. Since it was found out that legislation and terminology concerning the topic are not adequate, propositions were made. The thesis also deals with road safety which is influenced by drivers, vehicles and the environment and it includes the methods for its specification. By testing the hypotheses, it was realised that it is possible to influence the level of road safety if traffic-technical elements in multi-level intersections are chosen correctly. Finally, based on the analyses, suggestions for road condition improvement are put forward.