izpis_h1_title_alt

In vivo imaging of Lactococcus lactis, Lactobacillus plantarum and Escherichiacoli expressing infrared fluorescent protein in mice
Berlec, Aleš (Avtor), Štrukelj, Borut (Avtor), Završnik, Janja (Avtor), Turk, Boris (Avtor), Butinar, Miha (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.microbialcellfactories.com/content/pdf/s12934-015-0376-4.pdf Novo okno

Izvleček
Background: In vivo imaging of orally administered lactic acid bacteria (LAB) and commensal bacteria in mice is shown to provide information on the spatial and temporal distribution of bacteria in the gastrointestinal tract. The bacteria can be detected and monitored using bioluminescence or near-infrared fluorescence. Results: Fluorescence imaging of bacteria was established by expressing the infrared fluorescent protein IRFP713 in Lactococcus lactis, Lactobacillus plantarum and Escherichia coli. All three bacterial species were monitored in live mice and no major differences in transit time were observed. Bacteria passed through the stomach and small intestine in 1 h and the majority were secreted from the large intestine after 6-8 h. Intestinal localization of bacteria was confirmed by imaging the isolated intestines and culturing the intestinal content. The use of fluorescence tomography for spatial localization of fluorescent bacteria has been established. The expression of an additional infrared fluorescent protein IRFP682 enabled concomitant detection of two bacterial populations in live mice. Conclusions: The present work provides a methodological basis for future studies of probiotic and theranostic actions of LAB in mouse disease models.

Jezik:Angleški jezik
Ključne besede:in vivo imaging, lactic acid bacteria, Lactococcus lactis, Lactobacillus plantarum, Escherichia coli, infrared fluorescent protein, mice
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2015
Št. strani:str. 1-14
Številčenje:Vol. 14, no. 181
UDK:542:577.2
ISSN pri članku:1475-2859
DOI:10.1186/s12934-015-0376-4 Povezava se odpre v novem oknu
COBISS.SI-ID:3978609 Povezava se odpre v novem oknu
Število ogledov:536
Število prenosov:187
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
:
Objavi na: Bookmark and Share

Gradivo je del revije

Naslov:Microbial cell factories
Skrajšan naslov:Microb Cell Fact
Založnik:BioMed Central
ISSN:1475-2859
COBISS.SI-ID:2609172 Novo okno

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Komentarji

Dodaj komentar

Za komentiranje se morate prijaviti.

Komentarji (0)
0 - 0 / 0
 
Ni komentarjev!

Nazaj