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Detection, identification and genotyping of Borrellia spp. in rodents in Slovenia by PCR and culture
Avšič-Županc, Tatjana (Avtor), Ružić-Sabljić, Eva (Avtor), Cerar Kišek, Tjaša (Avtor), Korva, Miša (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.biomedcentral.com/1746-6148/11/188 Povezava se odpre v novem oknu

Izvleček
Background: Borrelia burgdorferi sensu lato, the agent of Lyme borreliosis, is mainly maintained in natural foci through the transmission cycles of competent tick vectors (Ixodes sp.) and a vertebrate reservoir. Specific rodents have been identified as the principal reservoir of Borrelia burgdorferi sensu lato in Europe. Borrelia miyamotoi is the only relapsing fever spirochete transmitted by the same tick. The aim of the present study was to perform an epidemiological survey to determine the presence of B. burgdorferi sensu lato in rodents occurring in Slovenia and to explore the presence of Borrelia miyamotoi. The study was performed in two parts, retrospective and prospective; a total of 297 rodents was analyzed. Detection and identification of borrelia was performed by molecular methods and additionally in the prospective study by isolation and genotyping (MluI-LRFP and MLST). Results: During the prospective part of the study, borrelia was isolated from 2/46 (4.3 %) lung specimens and from 10/46 (21.7 %) heart specimens of rodents. All isolated strains were identified as B. afzelii subtype Mla1, and MLST analysis revealed 5 distinct sequence types. Borrelia DNA was successfully detected by one or other of the PCR methods in 18/46 (39.1 %) and 75/251 (29.9 %) samples in the prospective and retrospective studies, respectively. LightMix% was found to be more sensitive than the %%in-house% nested PCR (91/297 (30.6 %) vs 48/297 (16.1 %)). Borrelia miyamotoi DNA was detected in 1/251 (0.4 %) and in 1/46 (2.2 %) heart specimens, in the retrospective and prospective parts of the study, respectively. Conclusion: We determined the prevalence of B. afzelii in rodents and report for the first time the presence of B. miyamotoi in Slovenia.

Jezik:Angleški jezik
Ključne besede:Borrelia burgdorferi sensu lato, rodents, molecular methods
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Leto izida:2015
Št. strani:str. 1-6
Številčenje:Vol. 11
UDK:579
ISSN pri članku:1746-6148
DOI:10.1186/s12917-015-0501-y Povezava se odpre v novem oknu
COBISS.SI-ID:32141529 Povezava se odpre v novem oknu
Število ogledov:452
Število prenosov:144
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Naslov:BMC veterinary research
Skrajšan naslov:BMC Vet Res
Založnik:BioMed Central
ISSN:1746-6148
COBISS.SI-ID:515674393 Povezava se odpre v novem oknu

Sekundarni jezik

Jezik:Neznan jezik
Ključne besede:Borrelia burgdorferi sensu lato, glodavci, molekularne metode

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