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Oleic acid metabolism via a conserved cytochrome P450 system-mediated omega-hydroxylation in the bark beetle-associated fungus Grosmannia clavigera
Stojan, Jure (Author), Komel, Radovan (Author), Šala, Martin (Author), Lah, Ljerka (Author), Novak, Metka (Author), Bohlmann, Joerg (Author)

URLURL - Presentation file, Visit http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0120119 This link opens in a new window

Abstract
The bark beetle-associated fungus Grosmannia clavigera participates in the large-scale de- struction of pine forests. In the tree, it must tolerate saturating levels of toxic conifer defense chemicals (e.g. monoterpenes). The fungus can metabolize some of these compounds through the ß-oxidation pathway and use them as a source of carbon. It also uses carbon from pine triglycerides, where oleic acid is the most common fatty acid. High levels of free fatty acids, however, are toxic and can cause additional stress during host colonization. Fatty acids induce expression of neighboring genes encoding a cytochrome P450 (CYP630B18) and its redox partner, cytochrome P450 reductase (CPR2). The aim of this work was to study the function of this novel P450 system. Using LC/MS, we biochemically characterized CYP630 as a highly specific oleic acid omega -hydroxylase. We explain oleic acid specificity using protein interaction modeling. Our results underscore the importance of omega - oxidation when the main ß-oxidation pathway may be overwhelmed by other substrates such as host terpenoid compounds. Because this CYP-CPR gene cluster is evolutionarily conserved, our work has implications for metabolism studies in other fungi.

Language:English
Keywords:oleic acid, metabolism, hydroxylation
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Year:2015
Numbering:Vol. 10, iss. 3
UDC:577
ISSN on article:1932-6203
DOI:10.1371/journal.pone.0120119 Link is opened in a new window
COBISS.SI-ID:31899353 Link is opened in a new window
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Downloads:425
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Record is a part of a journal

Title:PloS one
Publisher:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 This link opens in a new window

Secondary language

Language:Unknown
Keywords:oleinska kislina, presnova, hidroksilacija

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