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Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
Lojk, Jasna (Avtor), Pavlin, Mojca (Avtor), Erdani-Kreft, Mateja (Avtor), Veranič, Peter (Avtor), Pirkmajer, Sergej (Avtor), Bregar, Vladimir Boštjan (Avtor), Rajh, Maruša (Avtor), Miš, Katarina (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.dovepress.com/vishttp://www.dovepress.com/cell-type-specific-response-to-high-intracellular-loading-of-polyacryl-peer-reviewed-article-IJN Povezava se odpre v novem oknu

Izvleček
Magnetic nanoparticles (NPs) are a special type of NP with a ferromagnetic, electron-dense core that enables several applications such as cell tracking, hyperthermia, and magnetic separation, as well as multimodality. So far, superparamagnetic iron oxide NPs (SPIONs) are the only clinically approved type of metal oxide NPs, but cobalt ferrite NPs have properties suitable for biomedical applications as well. In this study, we analyzed the cellular responses to magnetic cobalt ferrite NPs coated with polyacrylic acid (PAA) in three cell types: Chinese Hamster Ovary (CHO), mouse melanoma (B16) cell line, and primary human myoblasts (MYO). We compared the internalization pathway, intracellular trafficking, and intracellular fate of our NPs using fluorescence and transmission electron microscopy (TEM) as well as quantified NP uptake and analyzed uptake dynamics. We determined cell viability after 24 or 96 hours' exposure to increasing concentrations of NPs, and quantified the generation of reactive oxygen species (ROS) upon 24 and 48 hours' exposure. Our NPs have been shown to readily enter and accumulate in cells in high quantities using the same two endocytic pathways; mostly by macropinocytosis and partially by clathrin-mediated endocytosis. The cell types differed in their uptake rate, the dynamics of intracellular trafficking, and the uptake capacity, as well as in their response to higher concentrations of internalized NPs. The observed differences in cell responses stress the importance of evaluation of NP-cell interactions on several different cell types for better prediction of possible toxic effects on different cell and tissue types in vivo.

Jezik:Angleški jezik
Ključne besede:magnetic nanoparticles, transmission electron microscopy, polyacrylic acid
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FE - Fakulteta za elektrotehniko
Leto izida:2015
Št. strani:str. 1449-1462
Številčenje:Vol. 10
UDK:577
ISSN pri članku:1178-2013
DOI:10.2147/IJN.S76134 Povezava se odpre v novem oknu
COBISS.SI-ID:31824089 Povezava se odpre v novem oknu
Število ogledov:1438
Število prenosov:266
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Gradivo je del revije

Naslov:International journal of nanomedicine
Založnik:Dove Medical Press
ISSN:1178-2013
COBISS.SI-ID:3223828 Povezava se odpre v novem oknu

Sekundarni jezik

Jezik:Neznan jezik
Ključne besede:magnetni nanodelci, prenosna elektronska mikroskopija, poliakrilne kisline

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