Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
Lojk, Jasna (Author), Pavlin, Mojca (Author), Erdani-Kreft, Mateja (Author), Veranič, Peter (Author), Pirkmajer, Sergej (Author), Bregar, Vladimir Boštjan (Author), Rajh, Maruša (Author), Miš, Katarina (Author)

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Magnetic nanoparticles (NPs) are a special type of NP with a ferromagnetic, electron-dense core that enables several applications such as cell tracking, hyperthermia, and magnetic separation, as well as multimodality. So far, superparamagnetic iron oxide NPs (SPIONs) are the only clinically approved type of metal oxide NPs, but cobalt ferrite NPs have properties suitable for biomedical applications as well. In this study, we analyzed the cellular responses to magnetic cobalt ferrite NPs coated with polyacrylic acid (PAA) in three cell types: Chinese Hamster Ovary (CHO), mouse melanoma (B16) cell line, and primary human myoblasts (MYO). We compared the internalization pathway, intracellular trafficking, and intracellular fate of our NPs using fluorescence and transmission electron microscopy (TEM) as well as quantified NP uptake and analyzed uptake dynamics. We determined cell viability after 24 or 96 hours' exposure to increasing concentrations of NPs, and quantified the generation of reactive oxygen species (ROS) upon 24 and 48 hours' exposure. Our NPs have been shown to readily enter and accumulate in cells in high quantities using the same two endocytic pathways; mostly by macropinocytosis and partially by clathrin-mediated endocytosis. The cell types differed in their uptake rate, the dynamics of intracellular trafficking, and the uptake capacity, as well as in their response to higher concentrations of internalized NPs. The observed differences in cell responses stress the importance of evaluation of NP-cell interactions on several different cell types for better prediction of possible toxic effects on different cell and tissue types in vivo.

Keywords:magnetic nanoparticles, transmission electron microscopy, polyacrylic acid
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FE - Faculty of Electrical Engineering
Number of pages:str. 1449-1462
Numbering:Vol. 10
ISSN on article:1178-2013
DOI:10.2147/IJN.S76134 Link is opened in a new window
COBISS.SI-ID:31824089 Link is opened in a new window
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Record is a part of a journal

Title:International journal of nanomedicine
Publisher:Dove Medical Press
COBISS.SI-ID:3223828 This link opens in a new window

Secondary language

Keywords:magnetni nanodelci, prenosna elektronska mikroskopija, poliakrilne kisline

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