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Combined liquid chromatography-tandem mass spectrometry analysis of progesterone metabolites
Sinreih, Maša (Avtor), Adamski, Jerzy (Avtor), Lanišnik-Rižner, Tea (Avtor), Sosič, Izidor (Avtor), Gobec, Stanislav (Avtor), Cesar, Jožko (Avtor), Zukunft, Sven (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0117984 Povezava se odpre v novem oknu

Izvleček
Progesterone has a number of important functions throughout the human body. While the roles of progesterone are well known, the possible actions and implications of progesterone metabolites in different tissues remain to be determined. There is a growing body of evidence that these metabolites are not inactive, but can have significant biological effects, as anesthetics, anxiolytics and anticonvulsants. Furthermore, they can facilitate synthesis of myelin components in the peripheral nervous system, have effects on human pregnancy and onset of labour, and have a neuroprotective role. For a better understanding of the functions of progesterone metabolites, improved analytical methods are essential. We have developed a combined liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detection and quantification of progesterone and 16 progesterone metabolites that has femtomolar sensitivity and good reproducibility in a single chromatographic run. MS/MS analyses were performed in positive mode and under constant electrospray ionization conditions. To increase the sensitivity, all of the transitions were recorded using the Scheduled MRM algorithm. This LC-MS/MS method requires small sample volumes and minimal sample preparation, and there is no need for derivatization. Here, we show the application of this method for evaluation of progesterone metabolism in the HES endometrial cell line. In HES cells, the metabolism of progesterone proceeds mainly to (20S)-20-hydroxy-pregn-4-ene-3-one, (20S)-20-hydroxy-5alpha-pregnane-3-one and (20S)-5alpha-pregnane-3alpha,20-diol. The investigation of possible biological effects of these metabolites on the endometrium is currently undergoing.

Jezik:Angleški jezik
Ključne besede:progesterone, chromatography, spectrometry
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Leto izida:2015
Številčenje:Vol. 10, iss. 2
UDK:616.4
ISSN pri članku:1932-6203
DOI:10.1371/journal.pone.0117984 Povezava se odpre v novem oknu
COBISS.SI-ID:31822297 Povezava se odpre v novem oknu
Število ogledov:798
Število prenosov:346
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Gradivo je del revije

Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Povezava se odpre v novem oknu

Sekundarni jezik

Jezik:Neznan jezik
Ključne besede:progesteron, kromatografija, spektrometrija

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