izpis_h1_title_alt

ICLIP predicts the dual splicing effects of TIA-RNA interactions
Wang, Zhen (Avtor), Kayikci, Melis (Avtor), Briese, Michael (Avtor), Zarnack, Kathi (Avtor), Luscombe, Nicholas M. (Avtor), Rot, Gregor (Avtor), Zupan, Blaž (Avtor), Curk, Tomaž (Avtor), Ule, Jernej (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1000530 Povezava se odpre v novem oknu

Izvleček
The regulation of alternative splicing involves interactions between RNA-binding proteins and pre-mRNA positions close to the splice sites. T-cell intracellular antigen 1 (TIA1) and TIA1-like 1 (TIAL1) locally enhance exon inclusion by recruiting U1 snRNP to 5' splice sites. However, effects of TIA proteins on splicing of distal exons have not yet been explored. We used UV-crosslinking and immunoprecipitation (iCLIP) to find that TIA1 and TIAL1 bind at the same positions on human RNAs. Binding downstream of 5' splice sites was used to predict the effects of TIA proteins in enhancing inclusion of proximal exons and silencing inclusion of distal exons. The predictions were validated in an unbiased manner using splice-junction microarrays, RT-PCR, and minigene constructs, which showed that TIA proteins maintain splicing fidelity and regulate alternative splicing by binding exclusively downstream of 5' splice sites. Surprisingly, TIA binding at 5' splice sites silenced distal cassette and variable-length exons without binding in proximity to the regulated alternative 3' splice sites. Using transcriptome-wide high-resolution mapping of TIA-RNA interactions we evaluated the distal splicing effects of TIA proteins. These data are consistent with a model where TIA proteins shorten the time available for definition of an alternative exon by enhancing recognition of the preceding 5' splice site. Thus, our findings indicate that changes in splicing kinetics could mediate the distal regulation of alternative splicing.

Jezik:Angleški jezik
Ključne besede:znotrajcelični antigen celic T, CLIP posameznih nukleotidov, uravnjavanje povezovanja, karta RNA
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FRI - Fakulteta za računalništvo in informatiko
Leto izida:2010
Št. strani:str. 1-16
Številčenje:Vol. 8, no. 10
UDK:004:575.112
ISSN pri članku:1544-9173
COBISS.SI-ID:8022356 Povezava se odpre v novem oknu
Število ogledov:417
Število prenosov:201
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
:
Objavi na:AddThis
AddThis uporablja piškotke, za katere potrebujemo vaše privoljenje.
Uredi privoljenje...

Gradivo je del revije

Naslov:PLoS biology
Skrajšan naslov:PLoS biol.
Založnik:Public Library of Science
ISSN:1544-9173
COBISS.SI-ID:512779289 Povezava se odpre v novem oknu

Sekundarni jezik

Jezik:Neznan jezik
Ključne besede:T-cell intracellular antigen, individual-nucleotide CLIP, splicing regulation, RNA map

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Komentarji

Dodaj komentar

Za komentiranje se morate prijaviti.

Komentarji (0)
0 - 0 / 0
 
Ni komentarjev!

Nazaj