izpis_h1_title_alt

iCLIP reveals the function of hnRNP particles in splicing at individual nucleotide resolution
König, Julian (Avtor), Zarnack, Kathi (Avtor), Rot, Gregor (Avtor), Curk, Tomaz (Avtor), Kayikci, Melis (Avtor), Zupan, Blaz (Avtor), Turner, Daniel J (Avtor), Luscombe, Nicholas M (Avtor), Ule, Jernej (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://eprints.fri.uni-lj.si/1147/ Novo okno

Izvleček
In the nucleus of eukaryotic cells, nascent transcripts are associated with heterogeneous nuclear ribonucleoprotein (hnRNP) particles that are nucleated by hnRNP C. Despite their abundance, however, it remained unclear whether these particles control pre-mRNA processing. Here, we developed individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to study the role of hnRNP C in splicing regulation. iCLIP data show that hnRNP C recognizes uridine tracts with a defined long-range spacing consistent with hnRNP particle organization. hnRNP particles assemble on both introns and exons but remain generally excluded from splice sites. Integration of transcriptome-wide iCLIP data and alternative splicing profiles into an 'RNA map' indicates how the positioning of hnRNP particles determines their effect on the inclusion of alternative exons. The ability of high-resolution iCLIP data to provide insights into the mechanism of this regulation holds promise for studies of other higher-order ribonucleoprotein complexes.

Jezik:Neznan jezik
Ključne besede:iCLIP splicing
Vrsta gradiva:Delo ni kategorizirano (r6)
Organizacija:FRI - Fakulteta za računalništvo in informatiko
Leto izida:2010
Št. strani:909-915
Številčenje:17
Število ogledov:322
Število prenosov:118
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
:
Objavi na: Bookmark and Share

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Komentarji

Dodaj komentar

Za komentiranje se morate prijaviti.

Komentarji (0)
0 - 0 / 0
 
Ni komentarjev!

Nazaj