Purification of GTP[gamma]S binding proteins from membranes of porcine brain using convective interaction media (CIM) supports
Bavec, Aljoša (Author), Podgornik, Aleš (Author), Zorko, Matjaž (Author)

URLURL - Presentation file, Visit http://www.dlib.si/details/URN:NBN:SI:doc-GLJYE5TB This link opens in a new window

Membranes from porcine brain bind relatively large quantities of guanosine 5ć-(3-0-thio) triphosphate (GTPgammaS), a poorly-hydrolysable analogue of GTP.Chrotnatography of solubilized proteins with 1% Sodium cholate using CIM DEAE column (Convective Interaction Media) revealed two fractions reach in GTPgammaS binding proteins. In reduced SDS-PAGE analysis only these two fractions showed a protein band with molecular mass 42 kDa which corresponded to alpha subunits Gs Gi or G11. Comparison between CIM DEAE and traditional DEAE-Sephacel methods showed that membrane bound heterotrimeric G - proteins might be isolated by HPLC using CIM supports on second or minute time scale.

Keywords:separation processes, chromatographic methods, CIM disks, GTP-gammaS, porcine brain
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Publisher:Slovensko kemijsko društvo
Number of pages:str. 371-379
Numbering:Vol. 47, no. 3
ISSN on article:1318-0207
COBISS.SI-ID:22653445 Link is opened in a new window
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Record is a part of a journal

Title:Acta chimica slovenica
Shortened title:Acta chim. slov.
Publisher:Slovensko kemijsko društvo, =Slovenian Chemical Society
COBISS.SI-ID:14086149 This link opens in a new window

Secondary language

Title:Čiščenje proteinov, ki vežejo GTP[gama]S, iz membran prašičjih možgan z uporabo CIM nosilcev
Keywords:biokemija, analizna kemija, separacijski procesi, proteini možganov, kromatografske metode, kromatografija, HPLC, CIM diski, heterotrimetrični G-proteini

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