izpis_h1_title_alt

Cross-affinity of peptide ligands selected from phage display library against pancreatic phospholipase A2 and ammodytoxin C
Gaser, Dominik (Author), Štrukelj, Borut (Author), Bratkovič, Tomaž (Author), Kreft, Samo (Author), Pungerčar, Jože (Author), Lunder, Mojca (Author)

URLURL - Presentation file, Visit http://acta.chem-soc.si/56/56-03-712.pdf This link opens in a new window

Abstract
Two phage-displayed random peptide libraries were screened for ligands with potential inhibitory activity against pancreatic phospolipase A2 or ammodytoxin C (neurotoxin found in the venom glands of Vipera ammodytes ammodytes). The interaction of selected peptides with pancreatic phospholipase A2 and ammodytoxin C was confirmed with surface plasmon resonance and phage ELISA assays. Interestingly, peptides showed equal affinity to both proteins, regardless which of the two proteins was used as a target in the selection procedure. Despite pronounced affinity, none of the synthetic peptides inhibited enzyme targets in vitro at the concentrations below 167 [micro]M.

Language:English
Keywords:pankreatična fosfolipaza A2, amoditoksin C, peptidni ligandi, bakteriofagi
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Year:2009
Publisher:Slovensko kemijsko društvo
Number of pages:str. 712-717
Numbering:Vol. 56, no. 3
UDC:577.2
ISSN on article:1318-0207
COBISS.SI-ID:2656113 Link is opened in a new window
Views:931
Downloads:173
Metadata:XML RDF-CHPDL DC-XML DC-RDF
 
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Record is a part of a journal

Title:Acta chimica slovenica
Shortened title:Acta chim. slov.
Publisher:Slovensko kemijsko društvo, =Slovenian Chemical Society
ISSN:1318-0207
COBISS.SI-ID:14086149 This link opens in a new window

Secondary language

Language:English
Abstract:
Pri iskanju ligandov s potencialno inhibitorno aktivnostjo na pankresno fosfolipazo A2 ali amoditoksin C (nevrotoksin iz strupnih žlez modrasa Vipera ammodytes ammodytes) smo uporabili dve knjižnici naključnih peptidov, izraženih na bakteriofagih. Peptidi, ki smo jih selekcionirali z različnimi selekcijskimi protokoli, jasno kažejo afiniteto do pankreasne fosfolipaze in amoditoksina C, ki smo jo določili s pomočjo površinske plazmonske resonance in encimskoimunskega testa. Peptidi presenetljivo izkazujejo podobno afiniteto do obeh proteinov, ne glede, katerega smo uporabili kot ciljno molekulo pri selekciji. Navkljub nedvoumni afiniteti, peptidi v območju testiranih koncentracij niso inhibirali fosfolipaznega delovanja in vitro.


Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back