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Hidrogelne obloge za vlažno celjenje ran s proteinsko učinkovino
Rošic, Romana (Author), Kristl, Julijana (Author), Baumgartner, Saša (Author)

URLURL - Presentation file, Visit http://www.sfd.si/modules/catalog/products/prodfile/fv_4_2010.pdf This link opens in a new window

Abstract
Sodoben pristop k celjenju ran temelji na vzdrževanju vlažnega mikrookolja na rani, kar lahko dosežemo s hidrogelnimi oblogami. Raziskave so pokazale, da lahko izboljšamo celjenje kroničnih ran z vključevanjem rastnih dejavnikov ali inhibitorjev proteaz v obloge. V članku je predstavljeno oblikovanje hidrogelov iz hidroksietilceluloze ali ksantana v različnih koncentracijah in z vgrajeno modelno proteinsko učinkovino. Hidrogeli iz 3% hidroksietilceluloze ali 6% ksantana imajo ustrezne reološke lastnosti, ne glede na uporabljen medij (voda, fosfatni pufer), sterilizacijo ali način zamrzovanja pred liofilizacijo. Uporaba blagih tehnoloških pogojev izdelave omogoča ohranitev nativne strukture proteina. Za preskuse sproščanja smo razvili lastno metodo, saj uveljavljeni modeli po farmakopejah ne posnemajo stanja v kronični rani. Metoda omogoča sproščanje proteina neposredno v medij brez vmesne bariere daljše časovno obdobje. Modelni protein se je iz hidroksietilceluloznega hidrogela sproščal difuzijsko nadzorovano, kar opisuje Higuchijeva kinetika, pri čemer se je v 4 dneh sprostilo 90 % vgrajenega proteina. Takšen hidrogel zagotavlja vlažno okolje na rani, vanj pabi lahko med drugim vgradili tudi proteazni inhibitor, ki bi pomagal povrniti ravnovesje med rastnimi dejavniki in proteazami v rani, kar bi zmanjšalo vnetni proces in omogočilo hitrejše celjenje.

Language:Slovenian
Keywords:hidrogel, reološke lastnosti, celjenje ran, modelni protein, kontrolirano sproščanje
Work type:Not categorized (r6)
Tipology:1.02 - Review Article
Organization:FFA - Faculty of Pharmacy
Year:2010
Publisher:Slovensko farmacevtsko društvo
Number of pages:str. 187-193
Numbering:Letn. 61, št. 4
UDC:66.02
ISSN on article:0014-8229
COBISS.SI-ID:2863729 Link is opened in a new window
Views:682
Downloads:122
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Record is a part of a journal

Title:Farmacevtski vestnik
Shortened title:Farm. vestn.
Publisher:Slovensko farmacevtsko društvo
ISSN:0014-8229
COBISS.SI-ID:3105794 This link opens in a new window

Secondary language

Language:English
Title:Protein loaded hydrogels as dressing for moist wound healing
Abstract:
Modern approach in the wound healing is based on maintenance of moist environment on the wound and as modern dressings hydrogels have been offered. Based on diffrent researches the healing of the wound can be improved by application of growth factors or proteasećs inhibitors. In the article the development of hydrogels from hydroxyethylcellulose or xantan in different concentrations and with incorporated model protein is presented. Hydrogels from 3% hydroxyethycellulose or 6% xantan posess appropriate rheological characteristics, regardless to the medium used (water, phosphate buffer), sterilization process or way of freezing before lyophilization. The mild technologic conditions of preparation prevent denaturation of the native structure of protein. A new method for release studies was developed, due to the fact that established pharmacopoeial models do not simulate chronic woundćs state. The method enables proteinćs release directly into the medium without barrier through longer period of time. The release of protein from hydroxyethylcellulose hydrogel is controlled by diffusion and in 4 days around90% of incorporated protein has been released. Hence, such hydrogel enables moist wound environment and incorporation of proteasećs inhibitor, which might help to regain the balance between growth factors and proteases inthe wound, what could decrease inflammation and guarantee faster healing.


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