Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate
Bregar, Vladimir Boštjan (Author), Lojk, Jasna (Author), Šuštar, Vid (Author), Veranič, Peter (Author), Pavlin, Mojca (Author)

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In recent years, nanoparticles (NPs) and related applications have become an intensive area of research, especially in the biotechnological and biomedical fields, with magnetic NPs being one of the promising tools for tumor treatment and as MRI-contrast enhancers. Several internalization and cytotoxicity studies have been performed, but there are still many unanswered questions concerning NP interactions with cells and NP stability. In this study, we prepared functionalized magnetic NPs coated with polyacrylic acid, which were stable in physiological conditions and which were also nontoxic short-term. Using fluorescence, scanning, and transmission electron microscopy, we were able to observe and determine the internalization pathways of polyacrylic acidcoated NPs in Chinese hamster ovary cells. With scanning electron microscopy we captured what might be the first step of NPs internalization an endocytic vesicle in the process of formation enclosing NPs bound to the membrane. With fluorescence microscopy we observed that NP aggregates were rapidly internalized, in a time-dependent manner, via macropinocytosis and clathrin-mediated endocytosis. Inside the cytoplasm, aggregated NPs were found enclosed in acidified vesicles accumulated in the perinuclear region 1 hour after exposure, where they stayed for up to 24 hours. High intracellular loading of NPs in the Chinese hamster ovary cells was obtained after 24 hours, with no observable toxic effects. Thus polyacrylic acidcoated NPs have potential for use in biotechnological and biomedical applications.

Keywords:magnetic nanoparticles, transmission electron, scanning electron microscopy
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Number of pages:str. 919-931
Numbering:Vol. 8
ISSN on article:1178-2013
COBISS.SI-ID:30476761 Link is opened in a new window
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Record is a part of a journal

Title:International journal of nanomedicine
Publisher:Dove Medical Press
COBISS.SI-ID:3223828 This link opens in a new window

Secondary language

Keywords:magnetni nanodelci, prenos elektronov, vrstični elektronski mikroskop

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