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Development and validation of dried blood spots technique for quantitative determination of topiramate using liquid chromatography-tandem mass spectrometry
Vnučec Popov, Tanja (Avtor), Cvitkovič-Maričič, Lea (Avtor), Prosen, Helena (Avtor), Brodnjak-Vončina, Darinka (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://onlinelibrary.wiley.com/doi/10.1002/bmc.2905/pdf Povezava se odpre v novem oknu

Izvleček
An LC-MS/MS method for determination of the anti-epileptic drug topiramate (TPM) in dried blood spot (DBS) samples was developed and validated. DBS samples were prepared by spotting 30 ŽL of spiked whole blood onto FTATM DMPK-C cards and drying for at least 3 h. Six-millimetre punched spots were then extracted by using a mixture of methanol and water (90:10, v/v) with deuterated internal standard (topiramate-d12). The extracted samples were injected into a liquid chromatograph equipped with a tandem mass spectrometric detector. Negative ions were monitored in the selected reaction monitoring mode and transitions m/z 338.2 8.1 and m/z 350.3 8.1 were used for the quantitative evaluation of TPM and internal standard, respectively. The results obtained from validation were statistically evaluated according to the requirements of the European Medicines Agency and US Food and Drug Administration regulatory guidelines. The linearity of the method was checked within a concentration range from 10 to 2000 ng/mL. The validation results indicate that the method is accurate, precise, sensitive, selective and reproducible.

Jezik:Angleški jezik
Ključne besede:topiramat, farmakokinetične študije, tekočinska kromatografija, masna spektrometrija, HPLC-MS/MS
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2013
Št. strani:str. 1054-1061
Številčenje:Vol. 27, iss. 8
UDK:543.2/.9
ISSN pri članku:0269-3879
DOI:10.1002/bmc.2905 Povezava se odpre v novem oknu
COBISS.SI-ID:3461745 Povezava se odpre v novem oknu
Število ogledov:902
Število prenosov:256
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Naslov:Biomedical chromatography
Skrajšan naslov:BMC, Biomed. chromatogr.
Založnik:Heyden & Son
ISSN:0269-3879
COBISS.SI-ID:1334138 Povezava se odpre v novem oknu

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