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Monitoring of imatinib targeted delivery in human leukocytes
Kralj, Eva (Avtor), Žakelj, Simon (Avtor), Trontelj, Jurij (Avtor), Pajič, Tadej (Avtor), Preložnik-Zupan, Irena (Avtor), Černelč, Peter (Avtor), Ostanek, Barbara (Avtor), Marc, Janja (Avtor), Kristl, Albin (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.sciencedirect.com/science/article/pii/S0928098713001693 Povezava se odpre v novem oknu

Izvleček
The success of imatinib therapy in chronic myeloid leukemia is highly influenced by its active transport into target cells. However, the methodology for analytical evaluation of intracellular drug concentration is rare and usually reliant upon the use of radioactively labeled drugs. More specifically, there is no published method available in the literature for the determination of imatinib concentration in granulocytes. To gain further insight into the intracellular drug uptake a very reliable two-stage sample concentration procedure was devised and coupled with a sensitive ultra-high performance liquid chromatography tandem mass spectrometry. The reliability of this sample preparation and sensitivity of the analysis was confirmed by a successful validation of all necessary method parameters to an impressive lower limit of quantification of 0.5 ng imatinib per 106 cells still at the signal to noise ratio of 670. The usefulness of the method is further improved with only 6 mL of blood being necessary for patient analysis. The method has been applied to blood samples of 13 CML patients treated with imatinib and all the measured intracellular drug concentrations were within the validated range. These and further measurements will enable the research of factors which may, besides blood plasma concentration, influence the individual's response to imatinib therapy. Furthermore, individualisation of dosing based on the directly measured targeted drug delivery could be possible.

Jezik:Angleški jezik
Ključne besede:imatinib, kronična levkemija, granulociti, zdravljenje
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.08 - Objavljeni znanstveni prispevek na konferenci
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2013
Št. strani:str. 123-129
Številčenje:Vol. 50, issue 1
UDK:615.2+616.15
ISSN pri članku:0928-0987
DOI:10.1016/j.ejps.2013.04.026 Povezava se odpre v novem oknu
COBISS.SI-ID:3441521 Povezava se odpre v novem oknu
Število ogledov:452
Število prenosov:102
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Gradivo je del zbornika

Naslov:Emerging Nanopharmaceuticals for Non-parenteral Application Routes
COBISS.SI-ID:3497073 Povezava se odpre v novem oknu

Gradivo je del revije

Naslov:European Journal of Pharmaceutical Sciences
Skrajšan naslov:Eur. j. pharm. sci.
Založnik:Elsevier
ISSN:0928-0987
COBISS.SI-ID:17553413 Povezava se odpre v novem oknu

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