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Neuroprotective role of [gama]-enolase in microglia in a mouse model of Alzheimer's disease is regulated by cathepsin X
Pišlar, Anja (Author), Glavan, Gordana (Author), Obermajer, Nataša (Author), Živin, Marko (Author), Schliebs, Reinhard (Author), Kos, Janko (Author)

URLURL - Presentation file, Visit http://onlinelibrary.wiley.com/doi/10.1111/acel.12093/abstract This link opens in a new window

Abstract
Gama-Enolase is a neurotrophic-like factor promoting growth, differentiation, survival and regeneration of neurons. Its neurotrophic activity is regulated by cysteine protease cathepsin X which cleaves the C-terminal end of the molecule. We have investigated the expression and co-localization of Gama-enolase and cathepsin X in brains of Tg2576 mice overexpressing amyloid precursor protein. In situ hybridization of gama-enolase and cathepsin X revealed that mRNAs for both enzymes were expressed abundantly around amyloid plaques. Immunostaining demonstrated that the C-terminally cleaved form of gama-enolase was present in the immediate plaque vicinity, whereas the intact form, exhibiting neurotrophic activity, was observed in microglia cells in close proximity to senile plaque. The upregulation of gama-enolase in microglial cells in response to amyloid-ß peptide (Aß) was confirmed in mouse microglial cell line EOC 13.31 and primary microglia and medium enriched with gama-enolase proved to be neuroprotective against Aß toxicity, however, the effect was reversed by cathepsin X proteolytic activity. These results demonstrate an upregulation of gama-enolase in microglia cells surrounding amyloid plaques in Tg2576 transgenic mice and demonstrate its neuroprotective role in amyloid-ß-related neurodegeneration.

Language:English
Keywords:gama enolaze, katepsin X, Alzheimerjeva bolezen, amiloid ß
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Year:2013
Number of pages:str. 604-614
Numbering:Vol. 12, iss. 4
UDC:547+577.2
ISSN on article:1474-9718
DOI:10.1111/acel.12093 Link is opened in a new window
COBISS.SI-ID:3441265 Link is opened in a new window
Views:836
Downloads:196
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Record is a part of a journal

Title:Aging cell
Shortened title:Aging cell
Publisher:Blackwell
ISSN:1474-9718
COBISS.SI-ID:512137241 This link opens in a new window

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