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Neuroprotective role of [gama]-enolase in microglia in a mouse model of Alzheimer's disease is regulated by cathepsin X
Pišlar, Anja (Avtor), Glavan, Gordana (Avtor), Obermajer, Nataša (Avtor), Živin, Marko (Avtor), Schliebs, Reinhard (Avtor), Kos, Janko (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://onlinelibrary.wiley.com/doi/10.1111/acel.12093/abstract Novo okno

Izvleček
Gama-Enolase is a neurotrophic-like factor promoting growth, differentiation, survival and regeneration of neurons. Its neurotrophic activity is regulated by cysteine protease cathepsin X which cleaves the C-terminal end of the molecule. We have investigated the expression and co-localization of Gama-enolase and cathepsin X in brains of Tg2576 mice overexpressing amyloid precursor protein. In situ hybridization of gama-enolase and cathepsin X revealed that mRNAs for both enzymes were expressed abundantly around amyloid plaques. Immunostaining demonstrated that the C-terminally cleaved form of gama-enolase was present in the immediate plaque vicinity, whereas the intact form, exhibiting neurotrophic activity, was observed in microglia cells in close proximity to senile plaque. The upregulation of gama-enolase in microglial cells in response to amyloid-ß peptide (Aß) was confirmed in mouse microglial cell line EOC 13.31 and primary microglia and medium enriched with gama-enolase proved to be neuroprotective against Aß toxicity, however, the effect was reversed by cathepsin X proteolytic activity. These results demonstrate an upregulation of gama-enolase in microglia cells surrounding amyloid plaques in Tg2576 transgenic mice and demonstrate its neuroprotective role in amyloid-ß-related neurodegeneration.

Jezik:Angleški jezik
Ključne besede:gama enolaze, katepsin X, Alzheimerjeva bolezen, amiloid ß
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2013
Št. strani:str. 604-614
Številčenje:Vol. 12, iss. 4
UDK:547+577.2
ISSN pri članku:1474-9718
DOI:10.1111/acel.12093 Povezava se odpre v novem oknu
COBISS.SI-ID:3441265 Povezava se odpre v novem oknu
Število ogledov:475
Število prenosov:115
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Gradivo je del revije

Naslov:Aging cell
Skrajšan naslov:Aging cell
Založnik:Blackwell
ISSN:1474-9718
COBISS.SI-ID:512137241 Novo okno

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