Mutagenicity and DNA damage of bisphenol A and its structural analogs

Fic, Anja (author), Žegura, Bojana (author), Sollner Dolenc, Marija (author), Filipič, Metka (author), Peterlin-Mašič, Lucija (author)

An environmental estrogen, bisphenol A (BPA), and its analogs are widespread in our living environment. Because their production and use are increasing, exposure of humans to bisphenols is becoming a significant issue. We evaluatedthe mutagenic and genotoxic potential of eight BPA structural analogs(BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1 and BP-2), with Ames and cometassays, respectively. None of the tested bisphenols (0.004, 0.02, 0.1 and0.5 mg/plate) showed a mutagenic effect in Salmonella typhimurium strains TA98 and TA100, in either the presence or absence of external S9-mediated metabolic activation (Aroclor 1254-induced male rat liver). The potential genotoxicity of bisphenols was determined in the human hepatoma cell line (HepG2) at non-cytotoxic concentrations (0.1 - 10 ŽM) after 4 and 24 h exposure. In the comet assay, BPA and its analog BPS induced significant DNA damage only after 24 h exposure, while analogs DMBPS, BP-1 and BP-2 induced transient increase in DNA strand breaks observed only after 4 h exposure. BPF,BPAF, BPZ and DMBPA did not induce DNA damage in the applied experimental conditions.

• Language: English
• Keywords: bisfenol A, bisfenoli, mutagenost, genotoksičnost, kometni test, Amesov test
• Work type: Not categorized (r6)
• Tipology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Year: 2013
• Number of pages: str. 189-200
• Numbering: Vol. 64, no. 2
• UDC: 615.9
• ISSN on article: 0004-1254
• DOI: 10.2478/10004-1254-64-2013-2319
• COBISS.SI-ID: 3437937
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Record is a part of a journal

• Title: Arhiv za higijenu rada i toksikologiju
• Shortened title: Arh. hig. rada toksikol.
• Publisher: Institut za medicinska istraživanja i medicinu rada
• ISSN: 0004-1254
• COBISS.SI-ID: 3833858

Secondary language

• Language: English
• Title: Mutagenost in poškodbe DNA povzročene z bisfenolom A in njegovimi strukturnimi analogi

Abstract

Okoljski estrogen, bisfenol A (BPA), in njegovi strukturni analogi so v velikimeri prisotni v našem okolju. Ker njihova proizvodnja in uporaba naraščata, je vse pomembneje ovrednotiti njihovo toksičnost zaradi izpostavljenosti ljudem. Z Amesovim in kometnim testom smo ovrednotili mutagenost in genotoksičnost osmih strukturnih analogov BPA (BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1 in BP-2). Nobeden od testiranih bisfenolov (0.004, 0.02, 0.1 in 0.5 mg/ploščo) ni izkazoval mutagenega delovanja na sevih TA98 in TA100 Salmonelle tryhimurium v prisotnosti in odsotnosti metabolne aktivacije (z Aroklorom 1254 inducirani encimi podganjih jeter). Potencialno genotoksičnost pa smo določali s kometnim testom na celični liniji humanega hepatoma(HepG2) pri necitotoksičnih koncentracijah (0.1 - 10 ŽM) po 4- in 24-urni izpostavljenosti. BPA in njegov analog BPS sta pri kometnem testu povzročila poškodbe samo po 24-urni izpostavljenosti, medtem ko so analogi DMBPS, BP-1 in BP-2 povzročili prehodne poškodbe DNA (samo po 4-urni izpostavljenosti). BPF, BPAF, BPZ in DMBPA niso povzročili poškodb DNA pri izbranih eksperimentalnih pogojih.