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Mutagenicity and DNA damage of bisphenol A and its structural analogs
Fic, Anja (Author), Žegura, Bojana (Author), Sollner Dolenc, Marija (Author), Filipič, Metka (Author), Peterlin-Mašič, Lucija (Author)

URLURL - Presentation file, Visit http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=152768 This link opens in a new window

Abstract
An environmental estrogen, bisphenol A (BPA), and its analogs are widespread in our living environment. Because their production and use are increasing, exposure of humans to bisphenols is becoming a significant issue. We evaluatedthe mutagenic and genotoxic potential of eight BPA structural analogs(BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1 and BP-2), with Ames and cometassays, respectively. None of the tested bisphenols (0.004, 0.02, 0.1 and0.5 mg/plate) showed a mutagenic effect in Salmonella typhimurium strains TA98 and TA100, in either the presence or absence of external S9-mediated metabolic activation (Aroclor 1254-induced male rat liver). The potential genotoxicity of bisphenols was determined in the human hepatoma cell line (HepG2) at non-cytotoxic concentrations (0.1 - 10 ŽM) after 4 and 24 h exposure. In the comet assay, BPA and its analog BPS induced significant DNA damage only after 24 h exposure, while analogs DMBPS, BP-1 and BP-2 induced transient increase in DNA strand breaks observed only after 4 h exposure. BPF,BPAF, BPZ and DMBPA did not induce DNA damage in the applied experimental conditions.

Language:English
Keywords:bisfenol A, bisfenoli, mutagenost, genotoksičnost, kometni test, Amesov test
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Year:2013
Number of pages:str. 189-200
Numbering:Vol. 64, no. 2
UDC:615.9
ISSN on article:0004-1254
DOI:10.2478/10004-1254-64-2013-2319 Link is opened in a new window
COBISS.SI-ID:3437937 Link is opened in a new window
Views:630
Downloads:146
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Record is a part of a journal

Title:Arhiv za higijenu rada i toksikologiju
Shortened title:Arh. hig. rada toksikol.
Publisher:Institut za medicinska istraživanja i medicinu rada
ISSN:0004-1254
COBISS.SI-ID:3833858 This link opens in a new window

Secondary language

Language:English
Title:Mutagenost in poškodbe DNA povzročene z bisfenolom A in njegovimi strukturnimi analogi
Abstract:
Okoljski estrogen, bisfenol A (BPA), in njegovi strukturni analogi so v velikimeri prisotni v našem okolju. Ker njihova proizvodnja in uporaba naraščata, je vse pomembneje ovrednotiti njihovo toksičnost zaradi izpostavljenosti ljudem. Z Amesovim in kometnim testom smo ovrednotili mutagenost in genotoksičnost osmih strukturnih analogov BPA (BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1 in BP-2). Nobeden od testiranih bisfenolov (0.004, 0.02, 0.1 in 0.5 mg/ploščo) ni izkazoval mutagenega delovanja na sevih TA98 in TA100 Salmonelle tryhimurium v prisotnosti in odsotnosti metabolne aktivacije (z Aroklorom 1254 inducirani encimi podganjih jeter). Potencialno genotoksičnost pa smo določali s kometnim testom na celični liniji humanega hepatoma(HepG2) pri necitotoksičnih koncentracijah (0.1 - 10 ŽM) po 4- in 24-urni izpostavljenosti. BPA in njegov analog BPS sta pri kometnem testu povzročila poškodbe samo po 24-urni izpostavljenosti, medtem ko so analogi DMBPS, BP-1 in BP-2 povzročili prehodne poškodbe DNA (samo po 4-urni izpostavljenosti). BPF, BPAF, BPZ in DMBPA niso povzročili poškodb DNA pri izbranih eksperimentalnih pogojih.


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