Bioactivation of bisphenol A and its analogs (BPF, BPAF, BPZ and DMBPA) in human liver microsomes
Schmidt, Jan (Author), Kotnik, Petra (Author), Trontelj, Jurij (Author), Knez, Željko (Author), Peterlin-Mašič, Lucija (Author)

URLURL - Presentation file, Visit http://www.sciencedirect.com/science/article/pii/S0887233313000490# This link opens in a new window

Bisphenol A analogs are a class of chemicals known as diphenylmethanes, which contain two benzene rings separated by one central carbon atom, usually with apara-hydroxy group on both benzene rings. Bisphenol A (BPA) can induce an uterotrophic response in immature CD-1 mice and elicits estrogenic responses in many other experimental systems. Besides highlighting endocrine effects, a number of metabolic studies provide strong support for the idea that reactive species of BPA are formed in vitro and in vivo that can form covalent adducts with nucleophilic macromolecules and/or produce oxidative stress. We used a liquid chromatography with a triple quadrupole tandem mass spectrometry (LC-MS/MS) for the detection of metabolites and glutathione conjugates of BPA and its analogs (BPF, BPAF, BPZ and DMBPA) in human liver microsomes (HLM) or with recombinant CYP isozymes in the presence of NADPH and GSH as a trapping agent. We have confirmed that BPA and its structural analogs form hydroxylated metabolites and electrophilic species during bioactivation in HLM and CYP isozymes. These results provided important mechanistic insight into the metabolic fate of BPA structural analogs in vitro.

Keywords:bisfenol A, LC-MS/MS, reaktivni metaboliti, jetrni mikrosomi, bisfenoli
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Number of pages:str. 1267-1276
Numbering:Vol. 27, issue 4
ISSN on article:0887-2333
DOI:10.1016/j.tiv.2013.02.016 Link is opened in a new window
COBISS.SI-ID:3408497 Link is opened in a new window
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Record is a part of a journal

Title:Toxicology in vitro
Shortened title:Toxicol. in vitro
COBISS.SI-ID:25596165 This link opens in a new window

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