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Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity
Berlec, Aleš (Avtor), Malovrh, Tadej (Avtor), Zadravec, Petra (Avtor), Steyer, Andrej (Avtor), Ravnikar, Matjaž (Avtor), Sabotič, Jerica (Avtor), Poljšak-Prijatelj, Mateja (Avtor), Štrukelj, Borut (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://link.springer.com/article/10.1007%2Fs00253-013-4722-3 Povezava se odpre v novem oknu

Izvleček
An epidemic shift in Hepatitis A virus (HAV) infection has been observed in recent years in rapidly developing countries, with increasing numbers of severe adult cases which has led to renewed interest in vaccination. Our approach in vaccine development uses recombinant expression of the highly immunogenic HAV antigen VP1-P2a in food-grade lactic acid bacterium Lactococcus lactis and in Escherichia coli. We used genetic constructs that enable nisin-controlled expression of the antigen in L. lactis in three different forms: (a) intracellularly, (b) on the bacterial surface and (c) on the bacterial surface fused with the fragment of the E. coli flagellin molecule that can act as a molecular adjuvant. Expression of the two surface forms of the antigen was achieved in L. lactis, and the resulting antigen-displaying bacteria were administered orally to mice. Half the animals in each of the two groups developed specific IgGs, with titers increasing over time and reaching 1:422 without flagellin and 1:320 with flagellin. A much higher titer 1:25,803 was observed with the parenterally administered antigen, which was purified from E. coli. With the latter, a significant mucosal IgA response was also observed. Despite significant titers, the IgGs elicited with oral or parenteral administration could not prevent HAV from infecting cells in a virus neutralization assay, suggesting that the antibodies cannot recognize viral surface epitopes. Nevertheless, orally administered HAV antigen expressed in L. lactis elicited significant systemic humoral immune response showing the feasibility for development of effective HAV vaccine for mucosal delivery.

Jezik:Angleški jezik
Ključne besede:hepatitis A, cepiva, imunski odziv, Lactococcus lactis, peroralna uporaba, Escherichia coli
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2013
Št. strani:str. 4333-4342
Številčenje:Vol. 97, iss. 10
UDK:615.371+616.36-08
ISSN pri članku:0175-7598
DOI:10.1007/s00253-013-4722-3 Povezava se odpre v novem oknu
COBISS.SI-ID:3398769 Povezava se odpre v novem oknu
Število ogledov:354
Število prenosov:130
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Naslov:Applied microbiology and biotechnology
Skrajšan naslov:Appl. microbiol. biotechnol.
Založnik:Springer
COBISS.SI-ID:5136135 Povezava se odpre v novem oknu

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