izpis_h1_title_alt

Peptides binding to the hunger hormone ghrelin
Vodnik, Miha (Avtor), Molek, Peter (Avtor), Štrukelj, Borut (Avtor), Lunder, Mojca (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite https://www.thieme-connect.com/ejournals/abstract/10.1055/s-0032-1331744 Novo okno

Izvleček
The neutralisation of circulating ghrelin, the only known peripheral orexigenic peptide hormone, is a promising approach for the pharmacological treatment of obesity. To select peptides with an affinity towards ghrelin, 4 selection procedures were carried out with random peptide phage display libraries Ph.D.-7 and Ph.D.-12. Due to the absence of a common consensus motif, a stepwise elimination approach was used. The pool of selected peptides displaying phage clones was thoroughly examined to remove any potential target-unrelated peptides. The affinity of the remaining phage clones for ghrelin was tested with ELISA. An analysis of the binding properties revealed four-phage displayed peptides that bind to ghrelin with moderate affinity, with ADTVPRH and MEMKKTHPVLGA being the most specific. Additional advantage of peptide MEMKKTHPVLGA is an indication of binding to octanoyl group on N-terminal part of ghrelin, involved in receptor interaction. Hence peptide MEMKKTHPVLGA represents the most suitable lead for further investigation.

Jezik:Angleški jezik
Ključne besede:ghrelin, des-acyl ghrelin, phage display, neutralising peptides
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2013
Št. strani:str. 372-377
Številčenje:Vol. 45, no. 5
UDK:577.2
ISSN pri članku:0018-5043
DOI:10.1055/s-0032-1331744 Povezava se odpre v novem oknu
COBISS.SI-ID:3398513 Povezava se odpre v novem oknu
Število ogledov:367
Število prenosov:107
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
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Gradivo je del revije

Naslov:Hormone and Metabolic Research
Skrajšan naslov:Horm. Metab. Res.
Založnik:Thieme-Stratton, Thieme
ISSN:0018-5043
COBISS.SI-ID:25570304 Novo okno

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