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The topography of electrospun nanofibers and its impact on the growth and mobility of keratinocytes
Pelipenko, Jan (Avtor), Kocbek, Petra (Avtor), Janković, Biljana (Avtor), Rošic, Romana (Avtor), Baumgartner, Saša (Avtor), Kristl, Julijana (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.sciencedirect.com/science/article/pii/S0939641112003074 Povezava se odpre v novem oknu

Izvleček
Despite a lot of intensive research in the field of polymer nanofibers as wound-healing and tissue-regeneration materials, the behavior of cells in contact with nanofibers in vitro as well as in vivo is still not well understood. However, this knowledge is crucial for the design of nanofibrillar materials that are suitable for biomedical applications. Therefore, in this study we present the preparation of poly(vinyl alcohol) (PVA) nanofibers from a physico-chemically characterized polymer solution by electrospinning together with a stabilization method to preserve the morphology of the nanofibers in aqueous conditions. An investigation of the effects of a nanofibrillar scaffold on the growth of human keratinocytes showed that randomly oriented PVA nanofibers delay the keratinocytesʼ adhesion but improve their strength, greatly alter their morphology, increase their metabolic activity and limit their mobility. We have shown that due to the small interfiber pores, the whole cells are unable to penetrate into nanofibrillar network efficiently. However, flexible cell parts can penetrate into the nanofibrillar network, whereas the cell nuclei stay on the surface of electrospun scaffold. Additional reason for poor cell mobility is random orientation of nanofibers, which does not provide continuous routes for successful cell infiltration. Therefore, nanofibrillar support with nanosized interfiber pores could potentially be used to enable an efficient cell proliferation and accelerate surface-wound healing, but not for 3D tissue regeneration. Finally, we showed that aligned nanofibers can successfully direct the migration and proliferation of cells, which is a crucial property of nanomaterials for the successful regeneration of tissues with a highly organized structure.

Jezik:Angleški jezik
Ključne besede:nanofibers, electrospinning, polyvinyl alcohol, keratinocytes, tissue regeneration, wound healing
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2013
Št. strani:str. 401-411
Številčenje:Vol. 84, iss. 2
UDK:542.9++543
ISSN pri članku:0939-6411
DOI:10.1016/j.ejpb.2012.09.009 Povezava se odpre v novem oknu
COBISS.SI-ID:3339121 Povezava se odpre v novem oknu
Število ogledov:694
Število prenosov:134
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Naslov:European journal of pharmaceutics and biopharmaceutics
Skrajšan naslov:Eur. j. pharm. biopharm.
Založnik:Wiss. Verl.-Ges.
ISSN:0939-6411
COBISS.SI-ID:13176839 Povezava se odpre v novem oknu

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