izpis_h1_title_alt

Three-dimensional invasion of macrophages is mediated by cysteine cathepsins in protrusive podosomes
Jevnikar, Zala (Avtor), Mirković, Bojana (Avtor), Pečar Fonović, Urša (Avtor), Zidar, Nace (Avtor), Švajger, Urban (Avtor), Kos, Janko (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://onlinelibrary.wiley.com/doi/10.1002/eji.201242610/pdf Povezava se odpre v novem oknu

Izvleček
Podosomes, specialized actin-rich structures in macrophages, degrade the extracellular matrix (ECM) and are involved in cell migration. On two-dimensional surfaces macrophages form spot-like podosomes at the ventral cell surface that develop into protrusive structures in a three-dimensional (3D) environment resembling the ECM. We have shown that the tips of these protrusive podosomes are characterized by increased accumulation of cysteine cathepsins (Cts) B, X, S, H and L, both in human blood macrophages and in human monocytic cell line U-937. Monocyte-to-macrophage differentiation induces an increase in cysteine cathepsin expression and activity, promoting their translocation to the cell surface, where they interact with ECM. This group of proteases is crucial for the extracellular as well as intracellular degradation of ECM, as demonstrated by quantitative monitoring of collagen IV degradation. Furthermore, inhibiting CtsB, X and S significantly impairs macrophage invasion through the 3D matrix. Time-lapse live-cell imaging of CtsB activity revealed that the extracellular and the intracellular ECM degradation are associated with extensive endocytosis at the tip of protrusive podosomes. The targeting of cysteine cathepsins, as the major mediators of human macrophage 3D invasion, could be an approach to the treatment of inflammatory and cancerous diseases.

Jezik:Angleški jezik
Ključne besede:makrofagi, cisteinski katepsini, podosomi, celične migracije, 3D matrice
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2012
Št. strani:str. 3429-3441
Številčenje:Vol. 42, no. 12
UDK:579.5+577.2
ISSN pri članku:0014-2980
DOI:10.1002/eji.201242610 Povezava se odpre v novem oknu
COBISS.SI-ID:3319921 Povezava se odpre v novem oknu
Število ogledov:437
Število prenosov:168
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
:
Objavi na:AddThis
AddThis uporablja piškotke, za katere potrebujemo vaše privoljenje.
Uredi privoljenje...

Gradivo je del revije

Naslov:European Journal of Immunology
Skrajšan naslov:Eur J Immunol
Založnik:Verlag Chemie GmbH, Academic Press
ISSN:0014-2980
COBISS.SI-ID:25429248 Povezava se odpre v novem oknu

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Komentarji

Dodaj komentar

Za komentiranje se morate prijaviti.

Komentarji (0)
0 - 0 / 0
 
Ni komentarjev!

Nazaj