izpis_h1_title_alt

The influence of differential processing of procathepsin H on its aminopeptidase activity, secretion and subcellular localization in human cell lines
Rojnik, Matija (Avtor), Jevnikar, Zala (Avtor), Doljak, Bojan (Avtor), Turk, Samo (Avtor), Zidar, Nace (Avtor), Kos, Janko (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.sciencedirect.com/science/article/pii/S0171933512000866# Novo okno

Izvleček
Cathepsin H is a unique member of the cysteine cathepsins that acts primarily as an aminopeptidase. Like other cysteine cathepsins, it is synthesized as an inactive precursor and activated by proteolytic removal of its propeptide. Here we demonstrate that, in human cells, the processing of the propeptide is an autocatalytic, multistep process proceeding from an inactive 41 kDa pro-form, through a 30 kDa intermediate form, to the 28 kDa mature form. Tyr87P and Gly90P were identified as the two major endopeptidase cleavage sites, converting the 30 kDa form into the mature 28 kDa form. The level of processing differs significantly in different human cell lines. In monocyte-derived macrophages U937 and prostate cancer cells PC-3, the 28 kDa form is predominant, whereas in osteoblasts HOS the processing from the 30 kDaform to the 28 kDa form is significantly lower. The aminopeptidase activityof the enzyme and its subcellular localization are independent of the product, however the 30 kDa form was not secreted in HOS cells. The activity of the resulting cathepsin H in U937 cells was significantly lower than that in HOS cells, presumably due to the high levels of endogenous cysteine protease inhibitor cystatin F present specifically in this cell line. These results provide an insight into the dependence of human cathepsin H processingand regulation on cell type.

Jezik:Angleški jezik
Ključne besede:katepsin H, celične linije, encimi, rak
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2012
Št. strani:str. 757-764
Številčenje:Vol. 91, iss. 10
UDK:616-006-097
ISSN pri članku:0171-9335
DOI:10.1016/j.ejcb.2012.05.004 Povezava se odpre v novem oknu
COBISS.SI-ID:3269745 Povezava se odpre v novem oknu
Število ogledov:697
Število prenosov:153
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Gradivo je del revije

Naslov:European journal of cell biology
Skrajšan naslov:Eur. j. cell biol.
Založnik:Elsevier, Urban u. Fischer
ISSN:0171-9335
COBISS.SI-ID:25426688 Novo okno

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