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A complex of genes involved in adaptation of Leptinotarsa decemlineata larvae to induced potato defense
Petek, Marko (Avtor), Turnšek, Neža (Avtor), Buh Gašparič, Meti (Avtor), Pompe Novak, Maruša (Avtor), Gruden, Kristina (Avtor), Slapar, Nina (Avtor), Popovič, Tatjana (Avtor), Štrukelj, Borut (Avtor), Jongsma, Maarten Anthonie (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://onlinelibrary.wiley.com/doi/10.1002/arch.21017/pdf Povezava se odpre v novem oknu

Izvleček
The Colorado potato beetle (Leptinotarsa decemlineata) is the most important pest of potato in many areas of the world. One of the main reasons for its success lies in the ability of its larvae to counteract plant defense compounds. Larvae adapt to protease inhibitors (PIs) produced in potato leaves through substitution of inhibitor-sensitive digestive cysteine proteases with inhibitor-insensitive cysteine proteases. To get a broader insight into the basis of larval adaptation to plant defenses, we created a "suppression subtractive hybridisation" library using cDNA from the gut of L. decemlineata larvae fed methyl jasmonate-induced or uninduced potato leaves. Four hundred clones, randomly selected from the library, were screened for their relevance to adaptation with DNA microarray hybridizations. Selected enzyme systems of beetle digestion were further inspected for changes in gene expression using quantitative PCR and enzyme activity measurements. We identified two new groups of digestive cysteine proteases, intestains D and intestains E. Intestains D represent a group of structurally distinct digestive cysteine proteases, of which the tested members are strongly upregulated in response to induced plant defenses. Moreover, we found that other digestive enzymes also participate in adaptation, namely, cellulases, serine proteases, and an endopolygalacturonase. In addition, juvenile hormone binding protein-like (JHBP-like) genes were upregulated. All studied genes were expressed specifically in larval guts. In contrast to earlier studies that reported experiments based on PI-enriched artificial diets, our results increase understanding of insect adaptation under natural conditions.

Jezik:Angleški jezik
Ključne besede:koloradski hrošč, genska ekspresija, encimi, insekti
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2012
Št. strani:str. 153-181
Številčenje:Vol. 79, no. 3
UDK:581.19
ISSN pri članku:0739-4462
COBISS.SI-ID:3232369 Povezava se odpre v novem oknu
Število ogledov:639
Število prenosov:279
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Naslov:Archives of insect biochemistry and physiology
Skrajšan naslov:Arch. insect biochem. physiol.
Založnik:Wiley-Liss
ISSN:0739-4462
COBISS.SI-ID:35402241 Povezava se odpre v novem oknu

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