Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell lines
Rojnik, Matija (Author), Jevnikar, Zala (Author), Mirković, Bojana (Author), Janeš, Damjan (Author), Zidar, Nace (Author), Kikelj, Danijel (Author), Kos, Janko (Author)

URLURL - Presentation file, Visit http://versita.metapress.com/content/p203104l6755v4x4/ This link opens in a new window

Background. Cathepsin H is a cysteine protease considered to play a major role in tumor progression, however, its precise function in tumorigenesis is unclear. Cathepsin H was recently proposed to be involved in processing of bone morphogenetic protein 4 (BMP-4) in mice. In order to clarify whether cathepsin H also regulates BMP-4 in humans, its impact on BMP-4 expression, processing and degradation was investigated in prostate cancer (PC-3), osteosarcoma (HOS) and pro-monocytic (U937) human cell lines. Materials and methods. BMP-4 expression was founded to be regulated by cathepsin H using PCR array technology and confirmed by real time PCR. Immunoassays including Western blot and confocal microscopy were used to evaluate the influence of cathepsin H on BMP-4 processing. Results. In contrast to HOS, the expression of BMP-4 mRNA in U937 and PC3 cells was significantly decreased by cathepsin H. The different regulation of BMP-4 synthesis could be associated with the absence of the mature 28 kDa cathepsin H form in HOS cells, where only the intermediate 30 kDa form was observed. No co-localization of BMP-4 and cathepsin H was observed in human cell lines and the multistep processing of BMP-4 was not altered in the presence of specific cathepsin H inhibitor. Isolated cathepsin H does not cleave mature recombinant BMP-4, neither with its amino- nor its endopeptidase activity. Conclusions. Our results exclude direct proteolytic processing of BMP-4 by cathepsin H, however, they provide support for its involvement in the regulation of BMP-4 expression.

Keywords:katepsin H, rak (medicina), proteolitični encimi, morfogenetični protein 4
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Number of pages:str. 259-266, II
Numbering:Vol. 45, no. 4
ISSN on article:1318-2099
DOI:10.2478/v10019-011-0034-3 Link is opened in a new window
COBISS.SI-ID:3157361 Link is opened in a new window
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Record is a part of a journal

Title:Radiology and oncology
Shortened title:Radiol. oncol.
Publisher:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
COBISS.SI-ID:32649472 This link opens in a new window

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