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Differences among Epilobium and Hypericum species revealed by four IR spectroscopy modes: transmission, KBr tablet, diffuse reflectance and ATR
Kokalj, Meta (Avtor), Kolar, Jana (Avtor), Trafela, Tanja (Avtor), Kreft, Samo (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://onlinelibrary.wiley.com/doi/10.1002/pca.1315/pdf Povezava se odpre v novem oknu

Izvleček
Quality control of dried plant material in assessments of suitability of herbal medicinal products is of extreme importance. Commonly used procedures for identification of species are time consuming and expensive. The development of multivariate statistical methods has enabled application of vibrational spectroscopy for establishing plant species membership. To determine which infrared spectroscopy mode gives most informative spectra for plant species identification. Methodology . Different modes of infrared spectroscopy were applied for investigation of differences among Epilobium and Hypericum species: diffuse reflectance (DR), attenuated total reflectance (ATR), direct transmission of whole leaves and KBr tablet transmission with comminuted leaves. The same chemometrical methods were applied to all spectra. The informative wave numbers were chosen by one ]way analysis of variance. Afterwards the colinearity was reduced with principal component analysis. In the final step the species identification was determined with discriminant analysis. Results . Transmission and diffuse reflectance mode did not give satisfactory results. Best results for discrimination among Epilobium species were obtained with ATR mode (98%), while best results for Hypericum species were obtained with KBr transmission mode (97%). This might be explained by the morphological properties of the two genera. Epilobium species differ in distribution and morphology of trichomes on the surface of the leaves. Hypericum species differ in structuresand secondary metabolites in the interior of the leaves. For best results in using infrared spectroscopy for plant species identification in quality control, the morphological properties of plant material should be taken into consideration.

Jezik:Angleški jezik
Ključne besede:KBr tablete, infrardeča spektroskopija, difuzna refleksija, herbarij vzorci
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2011
Št. strani:str. 541-546
Številčenje:Vol. 22, issue 6
UDK:542
ISSN pri članku:0958-0344
DOI:10.1002/pca.1315 Povezava se odpre v novem oknu
COBISS.SI-ID:3086449 Povezava se odpre v novem oknu
Število ogledov:909
Število prenosov:251
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Naslov:Phytochemical analysis
Skrajšan naslov:Phytochem. anal.
Založnik:Wiley
ISSN:0958-0344
COBISS.SI-ID:15074309 Povezava se odpre v novem oknu

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