A novel fluorescent probe for more effective monitoring of nanosized drug delivery systems within the cells
Teskač, Karmen (Author), Pajk, Stane (Author), Janković, Biljana (Author), Pečar, Slavko (Author), Srčič, Stanko (Author), Kristl, Julijana (Author)

URLURL - Presentation file, Visit http://www.sciencedirect.com/science/article/pii/S0378517311005990 This link opens in a new window

To improve visualization of nanoparticles within the cellsć compartments, we synthesized a coumarin based fluorescent derivative, tetradecyl diethylamino coumarin amid, 14-DACA. In this compound the coumarin chromophore is linked with a tetradecyl alkyl chain that contributes to lipophilicity and slightly amphiphilic character of this probe. 14-DACA exhibits good biocompatibility, its solubility and emission spectrum are not sensitive to changes in pH value.Solid lipid nanoparticles (SLN) labeled with 14-DACA (SLN-D) and frequently used 6-coumarin (SLN-C) were utilized to evaluate probesć properties in the trafficking and intracellular localization of nanoparticles.SLN-D were seen as distinct blue dots in the cellular environment in contrast to SLN-C which were hardly to recognize due to the self-quenching of 6-coumarin, its leakage and distribution in intracellular compartments. Spectra of 14-DACA indicated the possibility of spectral resolution from both green and red fluorophores allowing clear multicolor imaging of organelles in both fixed and living cells. The results showed valuableness of new probe for trafficking of the drug nanocarriers intracellularly in a kinetic and sensitive manner. Such studies are of great importance in investigations aimed to clarify subcellular targeted drug delivery, controlled release and even to identify toxicological changes.

Keywords:nanodelci, derivativi kumarina, dostavnost zdravil, nanotehnologija
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Number of pages:str. 384-393
Numbering:Vol. 416, no. 1
ISSN on article:0378-5173
COBISS.SI-ID:3075441 Link is opened in a new window
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Record is a part of a journal

Title:International journal of pharmaceutics
Shortened title:Int. j. pharm.
COBISS.SI-ID:3087631 This link opens in a new window

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