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Determination of raloxifene and its glucuronides in human urine by liquid chromatography-tandem mass spectrometry assay
Trdan Lušin, Tina (Avtor), Roškar, Robert (Avtor), Trontelj, Jurij (Avtor), Ravnikar, Matjaž (Avtor), Mrhar, Aleš (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.sciencedirect.com/science/article/pii/S1570023211004168 Novo okno

Izvleček
A selective, sensitive, accurate and precise liquid chromatography-tandem massspectrometry (LC-MS/MS) method for determination of raloxifene and its three glucuronides: raloxifene6 bglucuronide (M1), raloxifene4 0 bglucuronide (M2), raloxifene6,4 0diglucuronide (M3) in urine samples is presented in this paper. To our knowledge the developed analytical method is the first fully validated method capable of simultaneous determination of raloxifene and its glucuronides in real urine samples. Moreover, for the first time a method for determination of raloxifene diglucuronide in relevant biological samples was introduced. Metabolites were obtained by a bioconversion process of raloxifeneto its glucuronides using the microorganism Streptomyces sp. and were used as standards for validation. Urine samples were introduced to a simple solid phase extraction prior to the analysis by LC-MS/MS. The method was linear in a wide range with high determination coefficient (r2 > 0.997). The limits of quantification achieved were 1.01, 1.95, 2.83 and 4.69 nM for raloxifene, M1, M2 and M3, respectively. The recoveries were higher than 92.5%, the accuracy was within 100 8.8% and the precision was better than 12% for all compounds. The developed method was successfully applied to the real urine samples and showed to be appropriate for use in further research ofstill not completely discovered raloxifene pharmacokinetics. Furthermore, the presented method could also serve for a potential application in antidoping analysis.

Jezik:Angleški jezik
Ključne besede:raloksifen, urin, farmakokinetika, doping
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2011
Št. strani:str. 2323-2331
Številčenje:Vol. 879, issue 23
UDK:542+615.015
ISSN pri članku:1570-0232
DOI:10.1016/j.jchromb.2011.06.031 Povezava se odpre v novem oknu
COBISS.SI-ID:3063409 Povezava se odpre v novem oknu
Število ogledov:460
Število prenosov:100
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
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Gradivo je del revije

Naslov:Journal of chromatography
Založnik:Elsevier
ISSN:1570-0232
COBISS.SI-ID:2817144 Novo okno

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