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Modified phosphatidylserine-dependent antiprothrombin ELISA enables identification of patients negative for other antiphospholipid antibodies and also detects low avidity antibodies
Žigon, Polona (Author), Ambrožič, Aleš (Author), Čučnik, Saša (Author), Kveder, Tanja (Author), Rozman, Blaž (Author), Božič, Borut (Author)

URLURL - Presentation file, Visit http://www.reference-global.com/toc/cclm/49/6 This link opens in a new window

Abstract
Background: Two approaches for detecting anti-prothrombin antibodies have been described. The first detects antibodies against prothrombin alone and the second, phosphatidylserine- dependent antiprothrombin antibodies. The latter more often correlate with clinical manifestations of antiphospholipid syndrome and with lupus anticoagulant activity. Methods: In order to increase the capacity of antibody binding, we modified the previously described phosphatidylserine- dependent antiprothrombin ELISA and determined their avidity. We examined 203 patients with systemic autoimmune diseases and 222 blood donors. Results: Our modification resulted in a greater intensity of antibody binding to prothrombin on phosphatidylserine-coated plate surfaces compared to the previously described method. By changing ELISA conditions, we were able to detect with one assay the two, presumably different, populations of antiprothrombin antibodies. Diagnostic specificities of both ELISAs for antiphospholipid syndrome were similar (92.5% vs. 93.1%), while the sensitivity of the modified phosphatidylserine- dependent antiprothrombin ELISA was significantly higher than the anti-prothrombin alone ELISA (59% vs. 25%). Low avidity antiprothrombin antibodies were only detected in the modified phosphatidylserine-dependent antiprothrombin ELISA. Four percent of patients with positive phosphatidylserine-dependent antiprothrombin antibodies, showing clinical manifestations of antiphospholipid syndrome, were negative for all other antiphospholipid antibodies. The risk for antiphospholipid syndrome increased with the number of antiphospholipid antibody positivity. Conclusions: We conclude that antibodies detected with a modified phosphatidylserine-dependent antiprothrombin ELISA could improve the diagnosis of antiphospholipid syndrome by offering additional information on the risk for thrombosis, especially in patients negative for other antiphospholipid antibodies.

Language:English
Keywords:antifosfolipidna protitelesa, antiprotrombinska protitelesa, ELISA, lupus
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Year:2011
Number of pages:str. 1011-1018
Numbering:Vol. 49, no. 6
UDC:577
ISSN on article:1434-6621
DOI:10.1515/CCLM.2011.162 Link is opened in a new window
COBISS.SI-ID:3039857 Link is opened in a new window
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Downloads:241
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Record is a part of a journal

Title:Clinical chemistry and laboratory medicine
Shortened title:Clin Chem Lab Med
Publisher:Walter de Gruyter
ISSN:1434-6621
COBISS.SI-ID:1826580 This link opens in a new window

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