izpis_h1_title_alt

Gamma-1-syntrophin mediates trafficking of gamma-enolase towards the plasma membrane and enhances its neurotrophic activity
Pišlar, Anja (Avtor), Obermajer, Nataša (Avtor), Kos, Janko (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://content.karger.com/ProdukteDB/produkte.asp?Aktion=ShowAbstract&ArtikelNr=324292&Ausgabe=0&ProduktNr=224154 Povezava se odpre v novem oknu

Izvleček
Syntrophins are scaffold proteins that can bind several signaling molecules and localize them to the plasma membrane. We demonstrate here that in neuroblastoma SH-SY5Y cells, brain-specific gamma1-syntrophin binds the neurotrophic factor gamma-enolase through its PDZ domain, and translocates it to the plasma membrane, as shown by immunoprecipitation, surface plasmon resonance, fluorescence colocalization and flow cytometry. Extensive colocalization of gamma1-syntrophin and gamma-enolase was observed in neurite growth cones in differentiated SH-SY5Y cells. Silencing of the gamma1-syntrophin gene by RNA interference significantly reduced the re-distribution of gamma-enolase to theplasma membrane and impaired its neurotrophic effects. We demonstrated thatan intact C-terminal end of gamma-enolase is essential for its gamma1-syntrophin-assisted trafficking. The cleavage of two amino acids at C-terminal end of gamma-enolase by the carboxypeptidase cathepsin X prevents binding with the gamma1-syntrophin PDZ domain. Collectively, these data demonstrate that gamma1-syntrophin participates in gamma-enolase translocation towardsthe plasma membrane, a pre-requisite for its neurotrophic activity. By disrupting this gamma1-syntrophin-guided subcellular distribution, cathepsin X reduces gamma-enolase-induced neurotrophic signaling.

Jezik:Angleški jezik
Ključne besede:gamma-1-sintropin, gama enolaze, katepsin X, nevrotropična aktivnost
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2010
Št. strani:str. 246-258
Številčenje:Vol. 18, no. 4
UDK:577.2
ISSN pri članku:1424-862X
DOI:10.1159/000324292 Povezava se odpre v novem oknu
COBISS.SI-ID:2965617 Povezava se odpre v novem oknu
Število ogledov:586
Število prenosov:153
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
:
Objavi na:AddThis
AddThis uporablja piškotke, za katere potrebujemo vaše privoljenje.
Uredi privoljenje...

Gradivo je del revije

Naslov:NeuroSignals
Skrajšan naslov:NeuroSignals
Založnik:S. Karger
ISSN:1424-862X
COBISS.SI-ID:514941977 Povezava se odpre v novem oknu

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Komentarji

Dodaj komentar

Za komentiranje se morate prijaviti.

Komentarji (0)
0 - 0 / 0
 
Ni komentarjev!

Nazaj