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Probing the surface properties of solid lipid nanoparticles by atomic force microscopy
Janković, Biljana (Author), Teskač, Karmen (Author), Pajk, Stane (Author), Pečar, Slavko (Author), Srčič, Stanko (Author), Kristl, Julijana (Author)

URLURL - Presentation file, Visit http://www.scipharm.at/download.asp?id=753 This link opens in a new window

Abstract
Development of drug delivery systems is an indispensable strategy for successful transport of drug to its therapeutic site by the appropriate choiceof carrier and route. Solid lipid nanoparticles (SLN) include the advantages of conventional carriers and are additionally utilized for protection of labile compounds, as well as in controlling of drug release, targeting and stability. In general, physicochemical characteristics of SLN surface are those, which affect their behaviour both in vivo and in vitro [1, 2]. Consequently, development of appropriate design technique is crucial to effectively estimate the potential offered by SLN. The main objects of currentresearch were to evaluate nanoparticles morphology (size, distribution,shape, structure, integrity), and their nano-mechanical properties such as adhesion forces and local hardness. Atomic force microscopy(AFM) has been used for these studies. More precisely, phase imagingas an extension of the tapping mode AFM, provided the mapping of the surface properties variations, such as composition, adhesion, friction and visco-elasticity. SLN unlabelled and particles labelled with different coumarin based fluorescent dyes such as SPP 189 (SLN SPP-189) and 6-coumarine (SLN C), produced by melt-emulsification process were circular in shape. Photon correlation spectroscopy and AFM provided approximately the same particle size of investigated samples. Surface roughness of SLN SPP-189 was significantly elevated in comparison to unlabelled SLN, what should be the consequence of into surface incorporated fluorescent dye. Phase imaging and force spectroscopy measurement estimated the surface heterogeneity and local surface hardness. Phase contrast confirmed two different regions of SLN SPP- 189, which could be associated with surface heterogeneity caused by diverse nature of main constituents as well as fluorescent dye. Only these nanoparticles demonstrated light hallow in phase image, likely related to in homogeneous distribution of lipophilic ingredients that also reflects differences in local surface hardness. Current research will be additionally complemented with analysis of particlescells interaction in order to improve the understanding of SLN composition involved in the cell uptake and in cell drug delivery.

Language:English
Keywords:SLN, trdni lipidni nanodelci, zdravila
Work type:Not categorized (r6)
Tipology:1.12 - Published Scientific Conference Contribution Abstract
Organization:FFA - Faculty of Pharmacy
Year:2010
Number of pages:Str. 603
UDC:542:615
ISSN on article:0036-8709
COBISS.SI-ID:2860401 Link is opened in a new window
Views:452
Downloads:114
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Record is a part of a proceedings

Title:Abstracts of the 8th Central European Symposium on Pharmaceutical Technology (CESPT), Satellite Symposium: 4th International Graz Congress on Pharmaceutical Engineering, September 16th-18th, Graz, Austria
COBISS.SI-ID:2845297 This link opens in a new window

Record is a part of a journal

Title:Scientia Pharmaceutica
Shortened title:Sci. Pharm.
Publisher:Oesterreichische Apotheker-Verlagsgesellschat MBH
ISSN:0036-8709
COBISS.SI-ID:26371584 This link opens in a new window

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