Glutamate dehydrogenase activity in lymphocytes of B-cell chronic lymphocytic leukaemia patients
Pajič, Tadej (Author), Černelč, Peter (Author), Sešek-Briški, Alenka (Author), Lejko-Zupanc, Tatjana (Author), Malešič, Ivan (Author)

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Abstract Objectives: To investigate the pattern of glutamate dehydrogenase (GLDH) activity, GLUD1 and GLUD2 expressions in peripheral blood mononuclear cells (PBMC) of untreated B-chronic lymphocytic leukemia (B-CLL) in healthy individuals (HI) and patients with infectious mononucleosis (IM). Design and methods: GLDH activity was determined in a supernatant obtained from pelleted PBMC. GLUD1 and GLUD2 mRNA expression was determined using a quantitative real-time polymerase chain reaction. CD19+ B cells from PBMC were purified by using positive selection. Results: The highest GLDH activity was found in PBMC of the B-CLL group followed by the HI group and IM group. The PBMC GLDH activity was higher in 60% of the B-CLL patients according to the established reference interval for our HI (2.17-5.70 ?kat/g protein). The greater GLDH activity was also found in the CD19+ cell preparation of the B-CLL patients (two of the three) but not in HI (n = 3). The median value of GLUD1 expressionwas highest in the IM group (n = 11), followed by the HI (n = 14) and B-CLL groups (n = 59) (median 4.69/3.78, P < 0.005 and 4.69/2.91, P < 0.0005, respectively). GLUD2 expression was not significantly different between groups. Conclusions: The increased GLDH activity is specific for the PBMC of B-CLL patients. The GLUD1 but not the GLUD2 gene expression pattern is different between the PBMC of IM and B-CLL patients.

Keywords:glutamat dehidrogenaze, GLUD1, GLUD2, kronična limfocitna levkemija
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
Number of pages:str. 1677-1684
Numbering:Vol. 42, no. 16-17
ISSN on article:0009-9120
COBISS.SI-ID:2706801 Link is opened in a new window
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Title:Clinical biochemistry
Shortened title:Clin. biochem.
COBISS.SI-ID:22275033 This link opens in a new window

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