Glutamate dehydrogenase activity in lymphocytes of B-cell chronic lymphocytic leukaemia patients

Pajič, Tadej (author), Černelč, Peter (author), Sešek-Briški, Alenka (author), Lejko-Zupanc, Tatjana (author), Malešič, Ivan (author)

Abstract Objectives: To investigate the pattern of glutamate dehydrogenase (GLDH) activity, GLUD1 and GLUD2 expressions in peripheral blood mononuclear cells (PBMC) of untreated B-chronic lymphocytic leukemia (B-CLL) in healthy individuals (HI) and patients with infectious mononucleosis (IM). Design and methods: GLDH activity was determined in a supernatant obtained from pelleted PBMC. GLUD1 and GLUD2 mRNA expression was determined using a quantitative real-time polymerase chain reaction. CD19+ B cells from PBMC were purified by using positive selection. Results: The highest GLDH activity was found in PBMC of the B-CLL group followed by the HI group and IM group. The PBMC GLDH activity was higher in 60% of the B-CLL patients according to the established reference interval for our HI (2.17-5.70 ?kat/g protein). The greater GLDH activity was also found in the CD19+ cell preparation of the B-CLL patients (two of the three) but not in HI (n = 3). The median value of GLUD1 expressionwas highest in the IM group (n = 11), followed by the HI (n = 14) and B-CLL groups (n = 59) (median 4.69/3.78, P < 0.005 and 4.69/2.91, P < 0.0005, respectively). GLUD2 expression was not significantly different between groups. Conclusions: The increased GLDH activity is specific for the PBMC of B-CLL patients. The GLUD1 but not the GLUD2 gene expression pattern is different between the PBMC of IM and B-CLL patients.

• Language: English
• Keywords: glutamat dehidrogenaze, GLUD1, GLUD2, kronična limfocitna levkemija
• Work type: Not categorized (r6)
• Tipology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Year: 2009
• Number of pages: str. 1677-1684
• Numbering: Vol. 42, no. 16-17
• UDC: 616-006
• ISSN on article: 0009-9120
• COBISS.SI-ID: 2706801
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Record is a part of a journal

• Title: Clinical biochemistry
• Shortened title: Clin. biochem.
• Publisher: Elsevier.
• ISSN: 0009-9120
• COBISS.SI-ID: 22275033