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Targeting cancer cells using PLGA nanoparticles surface modified with monoclonal antibody
Kocbek, Petra (Avtor), Obermajer, Nataša (Avtor), Cegnar, Mateja (Avtor), Kos, Janko (Avtor), Kristl, Julijana (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%234944%239999%23999999999%2399999%23FLA%23&_cdi=4944&_pubType=J&view=c&_auth=y&_acct=C000033658&_version=1&_urlVersion=0&_userid=4776866&md5=ec399a09995ebfd1cebf3d37ef8dedf4 Povezava se odpre v novem oknu

Izvleček
Targeting drugs to their sites of action is still a major challenge in pharmaceutical research. In this study, polylactic-co-glycolic acid (PLGA) immuno-nanoparticles were prepared for targeting invasive epithelial breast tumour cells. Monoclonal antibody (mAb) was used as a homing ligand and was attached to the nanoparticle surface either covalently or non-covalently. The presence of mAb on the nanoparticle surface, its stability and recognition properties were tested. Protein assay, surface plasmon resonance, flow cytometry and fluorescence-immunostaining confirmed the presence of mAb on nanoparticles in both cases. However, a binding assay using cell lysate revealed that the recognition properties were preserved only for nanoparticleswith adsorbed mAb. These nanoparticles were more likely to be bound to the targeted cells than non-coated nanoparticles. Both types of nanoparticles entered the target MCF-10A neoT cells in mono-culture. In co-culture of MCF-10A neoT and Caco-2 cells immuno-nanoparticles were localized solely to MCF-10A neoT cells, whereas non-coated nanoparticles were distributed randomly. Immunonanoparticles entered only MCF-10A neoT cells, while non-coated nanoparticles were taken up by both cell types, indicating specific targeting of the immuno-nanoparticles. In conclusion, we demonstrate a method by which mAbs can be bound to nanoparticles without detriment to their targeting ability. Furthermore, the results show the effectiveness of the new carrier system for targeted delivery of small or large active substances into cells or tissues of interest.

Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2007
Št. strani:str. 18-26
Številčenje:Vol. 120, Iss. 1-2
UDK:615
ISSN pri članku:0168-3659
COBISS.SI-ID:2109297 Povezava se odpre v novem oknu
Število ogledov:524
Število prenosov:192
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Gradivo je del revije

Naslov:Journal of controlled release
Skrajšan naslov:J. control. release
Založnik:Elsevier
ISSN:0168-3659
COBISS.SI-ID:27318784 Povezava se odpre v novem oknu

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