izpis_h1_title_alt

Quantification of live and dead probiotic bacteria in lyophilised product by real-time PCR and by flow cytometry
Kramer, Mateja (Avtor), Obermajer, Nataša (Avtor), Bogovič Matijašić, Bojana (Avtor), Rogelj, Irena (Avtor), Kmetec, Vojko (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.springerlink.com/content/fg45527643638027/ Novo okno

Izvleček
The basic requirement for probiotic bacteria to be able to exert expected positive effects is to be alive; therefore, appropriate quantification methodsare crucial. Due to disadvantages of conventional microbiological methods, the bacterial quantification based on the nucleic acid detection is increasingly used. The objective of this study was to evaluate the possibilityto use propidium monoazide (PMA) in combination with real-time polymerase chain reaction (PCR) method or LIVE/DEAD BacLight viability kit in combination with flow cytometry (FCM) for determination of probiotic bacteria in a lyophilised product containing Lactobacillus acidophilus LA-5 and Bifidobacterium animalis ssp. lactis BB-12. In addition, the viability of probiotic bacteria in lyophilised product during 3 months storage was investigated. In the product, the results of real-time PCR quantification of PMA-treated cells did not differ significantly from those of non-treated cells, which indicate that most of the bacterial cells retained the membrane integrity although they have lost the culturability. The results obtained by FCM analysis were comparable with those by PMA real-time PCR. In conclusion, the PMA real-time PCR and FCM determination of the viability of probiotic bacteria could complement the plate count method which considers only the culturable part of the population.

Jezik:Angleški jezik
Ključne besede:probiotične bakterije, kvantifikacija, citometrija, tekočinska, nukleinska kislina
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FFA - Fakulteta za farmacijo
Leto izida:2009
Št. strani:str. 1137-1147
Številčenje:Vol. 84, no. 6
UDK:542:615
ISSN pri članku:0175-7598
DOI:10.1007/s00253-009-2068-7 Povezava se odpre v novem oknu
COBISS.SI-ID:2592625 Povezava se odpre v novem oknu
Število ogledov:522
Število prenosov:131
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
:
Objavi na: Bookmark and Share

Gradivo je del revije

Naslov:Applied microbiology and biotechnology
Skrajšan naslov:Appl. microbiol. biotechnol.
Založnik:Springer
COBISS.SI-ID:5136135 Novo okno

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Komentarji

Dodaj komentar

Za komentiranje se morate prijaviti.

Komentarji (0)
0 - 0 / 0
 
Ni komentarjev!

Nazaj