20.500.12556/RUL-99945 Tridimenzionalno biotiskanje kompleksnih tkiv Tridimensional bioprinting of complex tissue Tridimenzionalno biotiskanje je metoda tkivnega inženirstva, ki za natančno odlaganje celičnega materiala oziroma biočrnila uporablja tri načine. Biočrnila so v osnovi sestavljena iz vodne raztopine pred-polimerne zasnove in celic. Glavna naloga biočrnila je zagotavljanje mehanične podpore modelu, ki je v procesu tiskanja, obenem pa ustvari primerne razmere za celično preživetje in rast. Prvi način za izgradnjo tridimenzionalnega modela je tehnika izbrizga, ki temelji na treh načinih doziranja celic: pnevmatska, piezoelektrična in toplotno osnovana. Tehnika: laser uporablja za odmerjanje celic laserski žarek, ki usmerjeno pade na absorbcijsko plast titana ali železa, na spodnji strani pa nato odpade posamezen del biočrnila (celice) na absorbcijsko podlago. Tehnika potiska ima dva načina za odmerjanje pravilne količine biočrnila, in sicer pnevmatični ali hidravlični potisni bat. Vsekakor pa imajo opisani pristopi številne tehnične pomanjkljivosti. Zato bo potrebno v naslednjih letih izboljšati resolucijo tiskanja, hkratno razporejanje več različnih celičnih tipov, vaskularizacijo, mehanično trdnost tiskanega modela in zagotavljanje visokega preživetja celic. Trenutno se tridimenzionalni konstrukti že obsežno pojavljajo na področju regenerativne medicine za zagotavljanje presadkov in farmacevtske stroke za preizkušanje farmakokinetike zdravil. Three-dimensional bioprinting is a method of tissue engineering that uses three methods for precise deposition of cellular material or bioink. The bioinks are basically composed of an aqueous solution of pre-polymeric basis and cells. The main task of bioink is to provide mechanical support for the model that is in the process of printing, while creating the appropriate conditions for cell survival and growth. The first way to build a three-dimensional model is a technique of injection, based on three manners of cell dosing: pneumatic, piezoelectric and heat-based. Technique: laser uses laser beam for determining cells that falls targeted on the absorption layer of titanium or iron, and on the underside, then separate part of the bioink (cell) falls off on the absorption basis. The push technique has two methods of dosing the correct amount of bioink, namely a pneumatic or hydraulic push piston. In any case, the described approaches have many technical failures. Therefore, it will be necessary in the following years to improve the printing resolution, simultaneously allocating several different cell types, vascularization, mechanical strength of the printed model and ensuring the high cell survival. At the moment, three-dimensional constructs are already extensively appearing in the field of regenerative medicine for the provision of grafts and pharmaceutical fields for testing pharmacokinetics of medicines. biotechnology biotiskanje tridimenzionalno biotechnology bioprinting tridimensional true false false [S. Bohinc] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2018-02-24 07:15:16 2018-02-24 07:15:22 2022-08-24 17:11:43 0000-00-00 00:00:00 2017 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602:57.08:681.6-3(043.2) 150954 8932473 1234.pdf 1234.pdf 1 590B3702D5A024632B5AE9EB98F8266C e44236aee898cff1c912dd61ab93b0354d963daee2f62003f596d7bb8ae51b06 a875a8d0-a1b4-11eb-a523-00155dcfd717 20.500.12556/rul/7eb9d5a9-f2ce-4f33-a49c-f39f622a45c1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=109580 Biotehniška fakulteta 0 0 0