20.500.12556/RUL-95726 Vsebnost, lokalizacija in vezavne oblike železa v zrnih različnih sort pšenice CONTENT, LOCALIZATION AND BINDING FORMS OF IRON IN GRAINS OF DIFFERENT WHEAT CULTIVARS V magistrskem delu smo določali koncentracije Fe v zrnju različnih sort pšenice, določili njegova mesta akumulacije ter vezavne oblike. Koncentracije Fe smo določali s standardno rentgensko fluorescenčno spektrometrijo, z mikro-rentgensko fluorescenčno spektrometrijo smo lokalizirali Fe na tkivnem nivoju v zrnu, z mikro-rentgensko absorpcijsko spektrometrijo pa smo določili vezavne oblike Fe v celotnem zrnu in v posameznih tkivih zrna. Pričakovano so se sorte razlikovale po vsebnosti Fe. Največ ga je vsebovala sorta Super žitarka. Fe spojine, ki so ustrezale izmerjenim spektrom naših vzorcev, so bile pri vseh sortah enake: Fe2+ fitat, Fe3+ fitat in Fe3+ citrat. Tudi njihovi deleži, so bili pri vseh sortah primerljivi, z izjemo sorte Katarina, ki je vsebovala več Fe2+ fitata in manj Fe3+ citrata od ostalih sort, kar kaže na slabšo biodostopnost Fe pri omenjeni sorti. S Fe so najbolj bogata tkiva zrna: perikarp, nucleus in alevron. Perikarp ne vsebuje Fe3+ fitata, kar vpliva na večjo biorazpoložljivost Fe za rastline. Izstopa tudi embrio, kjer je bila povečana vsebnost Fe3+ citrata glede na Fe3+ fitat v primerjavi z ostalimi tkivi. Otrobi so pomemben vir Fe, vendar se v predelavi za prehrambene namene izgubijo. Njihova uporaba bi pomenila pomemben korak v smeri biofortifikacije živil. In the dissertation, we have been determining Fe concentration in grain of various sorts of wheat, its places of accumulation, and its binding forms. We have been determining the Fe concentrations with Standard Energy-Dispersive X-ray Fluorescence Spectrometry, the localization of Fe on tissue level of grain with Micro X-ray Absorption Spectroscopy and Fe binding forms in the whole grain and in separate tissues with Micro- X-ray Fluorescence Spectroscopy. As expected, various sorts differed in Fe content, with the sort »Super žitarka« containing the most Fe. Fe compounds that have corresponded to our measured spectres have been the same in all sorts: Fe2+ phytate, Fe3+ phytate and Fe3+ citrate. Their shares have also been comparable in all sorts, with the exception of the sort »Katrina« that contained more Fe2+ phytate and less Fe3+ citrate than other sorts, which shows inferior bioaccessibility of Fe in the said sort. The tissues most rich in Fe are the pericarp, nucleus and alveron. The pericarp does not contain Fe3+ phytate, which effects the greater bioaccessibility of Fe. The embryo also stands out with the increased content of Fe3+ citrate according to the Fe3+ phytate in comparison with the other tissues. For biofortification, it would be particularly necessary to use wheat bran that are rich in Fe and are lost in the processing. železo/pšenica/vsebnost/lokalizacija/vezavne oblike/fortifikacija/rentgenske analitske metode iron/wheat/content/localization/binding form/fortification/X-Ray analitical tools true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2017-09-21 07:58:08 2017-09-21 07:58:08 2022-08-10 06:47:40 0000-00-00 00:00:00 2017 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 144697 1402.pdf 1402.pdf 1 ACB54AE216FBB5319B98F6C81EF1B5A6 e42dbb38d20831e04e9f504ceb051b7af1c74b4fed2f412ad8371b5971d72b2a d4a01e04-a1b3-11eb-a523-00155dcfd717 20.500.12556/rul/f734cb17-022f-4d38-ba48-afc5e7c925b9 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=104357 Biotehniška fakulteta 0 0 0