20.500.12556/RUL-95651 Interakcije polifenolov z encimi prebavnega trakta interactions of polyphenols with dygestive enzymes Strukturne značilnosti polifenolnih spojin in njihov vpliv na inhibicijo encimov prebavnega trakta s poudarkom na α-glukozidazi, α-amilazi, pepsinu, tripsinu, lipazi in kimotripsinu. Znano je, da polifenoli tvorijo komplekse s proteini, kar vodi do sprememb v strukturnih, funkcionalnih in prehranskih lastnostih obojh. Molekulska masa, število in položaj substitucij ter glikozilacija flavonoidov so povezani z inhibitornim učinkom polifenolov na encime. Učinek se razlikuje med encime, ki hidrolizirajo ogljikove hidrate, in med proteazami in lipazami. Interakcije med encimi in polifenoli so regulirane z nekovalentnimi vezmi, večinoma z van der Waalsovimi vezmi, vodikovimi vezmi in hidrofobnimi ter elektrostatičnimi interakcijami. Vezava polifenolnih spojin nekompetitivno inhibira encimske aktivnosti. The present work focuses on PCs structural characteristics behind the inhibition of digestive enzymes, emphasizes on the digestive enzymes as α-glycosidase. α-amylase, lipase, pepsin, trypsin , and chymotrypsin. Polyphenols are known to form complexes with proteins leading to changes in the structural, functional and nutritional properties of both compounds. Some characteristics such as molecular weight, number and position of substitution, and glycosylation of flavonoids seem to be related to the inhibitory effect of polyphenolss; also, this effect seems to be different for carbohydrate-hydrolyzing enzymes and proteases and lipases. The digestive enzyme–polyphenols molecular interactions have shown that non-covalent binding, mostly by van der Waals forces, hydrogen binding, hydrophobic binding, and other electrostatic forces regulate them. These interactions were mainly associated to non-competitive type inhibitions of the enzymatic activities. polifenoli encimi interakcije prebavni trakt glikozilacija polyphenols enzymes interactions digestion glycosylati true false false [K. Pišotek] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2017-09-21 07:53:46 2017-09-21 07:53:46 2022-08-10 06:40:45 0000-00-00 00:00:00 2017 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 604.4:577.15(043.2) 143865 8809593 1338.pdf 1338.pdf 1 708CA3E0CD965C3D768BAF33B4A84DFE 915a1e48d9d466b47d28d66e513998105e67c6b18400954d460d4515994e21ba cfb5cbaf-a1b3-11eb-a523-00155dcfd717 20.500.12556/rul/4dbc2cfd-dd6b-424b-9ff2-4a67784a9390 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=104277 Biotehniška fakulteta 0 0 0